PPIRE17697

Target Protein Information
Protein_Name None
Protein_Sequence MQADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK
Organism_Source Bos taurus
Functional_Classification EF hand Ca2+ binding proteins
Cellular_Localization Cytoplasm
Gene_Names CALM1
UniProt_ID A0AAF6ZWW2
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name Peptide 13 (F19A)
Peptide_Sequence CAAARWKKNFIAVSAANRAKKIS
Peptide_Length 23
Peptide_SMILES CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@H](C)NC(=O)[C@@H](N)CS)C(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)O)[C@@H](C)CC)C(C)C
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Amide
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 2504.98
Aliphatic_Index 76.95652
Aromaticity 0.08696
Average_Rotatable_Bonds 3.69565
Charge_at_pH_7 5.93482
Isoelectric_Point 11.82297
Hydrogen_Bond_Acceptors 35
Hydrogen_Bond_Donors 40
Topological_Polar_Surface_Area 1073.83000
X_logP_energy -11.37416
Interaction Information
Affinity KD=0.9 nM
Affinity_Assay Surface Plasmon Resonance
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Alanine substitutions in calmodulin-binding peptides result in unexpected affinity enhancement
Release_Year 1996
PMID 8613992
DOI 10.1006/jmbi.1996.0229