PPIRE18100

Target Protein Information
Protein_Name Guanylate cyclase
Protein_Sequence MPGPQHSTGARQCLLLFLLLLLRGSHARNLTVAVVLPLANTSYPWSWARVGPAVELALARVQARRDALPGWTVRTVLGSSENALGACSDTAAPLAAVDLKWEHKPAVFLGPGCVYAAAPVGRFTAHWRVPLLTAGAPALGFGSKDEYALTTRSGPSHAKLGDFVAALHRRLGWESRALVLYAYRPGDDQPCFFVVEGLYVRVRDRLNITVDHMEFAEGDRDHYTLLLRTVRSKGRVIYICSSPDAFRTLMLLALDAGLSGEDYVFFHLDIFGQSLQGAGAPAARRPWERGDGRDVSAQQAFQAAKIITYKEPDNPEYLEFLTQLKHLALEQFNFTVEDGLVNIIPASFHDGLLLYLQAVAETLAQGGTVTDGEDITQRMWNRSFQGVMGYLKMDSNGDRDTDFSLWDMDPETGAFQVALNYNGTSQELLAVSGRKLYWPLGYPPPDIPKCGFDNEDPACNQDHFSTLEVLAVVGSISLLSILIVSFFIYRKMQLEKELASELWRVRWEDLQPSSLERHLRSTGSRLTLSGRGSNYGSLLTTEGQFQIFAKTAYYKMRDVQNEHLTRFVGACTDPPNICILTEYCPRGSLQDILENESITLDWMFRYSLTNDIVKGMLFLHNGAICSHGNLKSSNCVVDGRFVLKITDYGLESFRDPEPEQGHTIYAKKLWTAPELLRMASPPARGSQAGDIYSFGIILQEIALRSGVFHVEGLDLSPKEIIERVTRGEQPPFRPSLALQSHMEELGQLMQRCWAEDPQERPPFQQIRLTLRKFNRENSSNILDNLLSRMEQYANNLEELVEERTQAYLEEKRKAEALLYQILPHSVAEQLKRGETVQAEAFDSVTIYFSDIVGFTALSAESTPMQVVTLLNDLYTCFDAVIDNFDVYKVETIGDAYMVVSGLPVRNGRLHAREVARMALALLDAVRSFRIRHRPQEQLRLRIGIHTGPVCAGVVGLKMPRYCLFGDTVNTASRMESNGEALKIHLSSETKAVLEEFEGFELELRGDVEMKGKGKLHHCPGIGEVV
Organism_Source Oryctolagus cuniculus
Functional_Classification guanylyl cyclase receptors
Cellular_Localization Plasma membrane
Gene_Names NPR1
UniProt_ID A0A5F9CD36
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name Recombinant atrial natriuretic peptide (rANP)
Peptide_Sequence SPSSRSCFGGRMDRIGAQSGLGCNSFRY
Peptide_Length 28
Peptide_SMILES CC[C@H](C)[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCSC)NC(=O)[C@H](CCCNC(=N)N)NC(=O)CNC(=O)CNC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CS)NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CO)NC(=O)[C@H](CO)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CO)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)NCC(=O)N[C@@H](CS)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)O
Chemical_Modification None
Cyclization_Method side chain-side chain cyclization; C7<-->C23; disulfide bond
Linear/Cyclic Cyclic
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 2997.33
Aliphatic_Index 31.42857
Aromaticity 0.10714
Average_Rotatable_Bonds 3.50000
Charge_at_pH_7 2.87362
Isoelectric_Point 9.59233
Hydrogen_Bond_Acceptors 46
Hydrogen_Bond_Donors 52
Topological_Polar_Surface_Area 1352.92000
X_logP_energy -21.06792
Interaction Information
Affinity IC50=19 nM
Affinity_Assay isometric tension assay
PDB_ID None
Type Agonist
Structure
Reference Information
Document_Type Research Articles
Title Synthesis of recombinant atrial natriuretic peptide (rANP) using hybrid fusion protein-phage fr coat/ANP (CP/ANP)
Release_Year 1997
PMID 9396066
DOI 10.1016/s0196-9781(97)00148-4