PPIRE18318
Target Protein Information
| Protein_Name | Replication protein A 70 kDa DNA-binding subunit |
|---|---|
| Protein_Sequence | MVGQLSEGAIAAIMQKGDTNIKPILQVINIRPITTGNSPPRYRLLMSDGLNTLSSFMLATQLNPLVEEEQLSSNCVCQIHRFIVNTLKDGRRVVILMELEVLKSAEAVGVKIGNPVPYNEGLGQPQVAPPAPAASPAASSRPQPQNGSSGMGSTVSKAYGASKTFGKAAGPSLSHTSGGTQSKVVPIASLTPYQSKWTICARVTNKSQIRTWSNSRGEGKLFSLELVDESGEIRATAFNEQVDKFFPLIEVNKVYYFSKGTLKIANKQFTAVKNDYEMTFNNETSVMPCEDDHHLPTVQFDFTGIDDLENKSKDSLVDIIGICKSYEDATKITVRSNNREVAKRNIYLMDTSGKVVTATLWGEDADKFDGSRQPVLAIKGARVSDFGGRSLSVLSSSTIIANPDIPEAYKLRGWFDAEGQALDGVSISDLKSGGVGGSNTNWKTLYEVKSENLGQGDKPDYFSSVATVVYLRKENCMYQACPTQDCNKKVIDQQNGLYRCEKCDTEFPNFKYRMILSVNIADFQENQWVTCFQESAEAILGQNAAYLGELKDKNEQAFEEVFQNANFRSFIFRVRVKVETYNDESRIKATVMDVKPVDYREYGRRLVMSIRRSALM |
| Organism_Source | Homo sapiens |
| Functional_Classification | single stranded DNA binding proteins |
| Cellular_Localization | Nucleus |
| Gene_Names | RPA1 |
| UniProt_ID | P27694 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | BLM153-165 |
|---|---|
| Peptide_Sequence | DWDDMDDFDTSET |
| Peptide_Length | 13 |
| Peptide_SMILES | CSCC[C@H](NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@@H](N)CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@H](C(=O)O)[C@@H](C)O)[C@@H](C)O |
| Chemical_Modification | None |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | FITC-6-aminohexanoic acid |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 1591.53 |
|---|---|
| Aliphatic_Index | 0.00000 |
| Aromaticity | 0.15385 |
| Average_Rotatable_Bonds | 3.84615 |
| Charge_at_pH_7 | -6.99756 |
| Isoelectric_Point | 3.02815 |
|---|---|
| Hydrogen_Bond_Acceptors | 25 |
| Hydrogen_Bond_Donors | 25 |
| Topological_Polar_Surface_Area | 750.10000 |
| X_logP_energy | -8.59740 |
Interaction Information
| Affinity | KD=5.76 uM |
|---|---|
| Affinity_Assay | Fluorescence Polarization |
| PDB_ID | None |
| Type | Inhibitor |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Interaction of replication protein A with two acidic peptides from human Bloom syndrome protein |
| Release_Year | 2019 |
| PMID | 29388204 |
| DOI | 10.1002/1873-3468.12992 |