PPIRE18522
Target Protein Information
| Protein_Name | Guanylate cyclase |
|---|---|
| Protein_Sequence | MVPAEAMPRPGRPSGARLRLLLLLPPLLLLPGSYAGNLTVAVVLPLTNTSYPWSWARVGPAVELALARVKARPDLLPGWTVRTVLGSSESALGVCSDTAAPLAAVDLKWEHSPAVFLGPGCVYAAAPVGRFTAHWRVPLLTAGAPAVGFGAKDEYALTTRAGPSHAKLGDFVAALHRRLGWERQALVLYAYQPGDDQPCFFVVEGLYMRVRDRLNITVDHLEFAEGDRDHYTMLLRTVRRKGRVIYICSSPDTFRALMLLALEAGLSGNDYVFFHLDLFGQSLQGAHGIAPRRPWERGDGQDVSAHQAFRAAKIITYKEPDNPEYLEFLQKLKRLALEQFNFTMEDGLVNTIPASFHDGLLLYIQAVTETLAHGGAVTDGEGITQRMWNRSFQGVTGYLKMDSNGDRETDFSLWDMDPNTGAFRVVLNFNGTSQELMTMSGCKLNWPLGYPPPDVPKCGFDNEDPACNQDHFSTLEVLALVGSLSLLSILIVSFFIYRKMQLEKELASELWRVRWEDLQPSSLERHLRSAGSRLTLSGRGSNYGSLLTTEGQLQIFAKTAYYKGNLVAVKRVNRKRIELTREVLFELKHMRDVQNEHLTRFVGACTDPPNICILTEYCPRGSLQDILENESITLDWMFRYSLTNDIVKGMLFLHNGAICSHGNLKSSNCVVDGRFVLKITDYGLESFRDPEPEQGHTLYAKKLWTAPELLRMASPPARGSQAGDVYSFGIILQEIALRSGAFHVEGLDLSPKEIIERVTRGEQPPFRPSLALQSHLEELGQLMQRCWAEDPQERPPFQQIRLMLRKFNRENSSNILDNLLSRMEQYANNLEELVEERTQAYLEEKRKAEALLYQILPHSVAEQLKRGETVQAEAFDSVTIYFSDIVGFTALSAESTPMQVVTLLNDLYTCFDAVIDNFDVYKVETIGDAYMVVSGLPVRNGRLHAREVARMALALLDAVRSFRIRHRPQEQLRLRIGIHTGPVCAGVVGLKMPRYCLFGDTVNTASRMESNGEALKIHLSSETKAVLEEFGGFELELRGDVEMKGKGKVRTYWLLGERGSSTRG |
| Organism_Source | Bos taurus |
| Functional_Classification | receptor guanylyl cyclases |
| Cellular_Localization | Plasma membrane |
| Gene_Names | NPR1 |
| UniProt_ID | E1BN71 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | Anantin |
|---|---|
| Peptide_Sequence | GFIGWGNDIFGHYSGDF |
| Peptide_Length | 17 |
| Peptide_SMILES | CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)CN)C(=O)NCC(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@H](C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)O)[C@@H](C)CC |
| Chemical_Modification | None |
| Cyclization_Method | Main chain-side chain cyclization; G1<-->D8; amide bond |
| Linear/Cyclic | Cyclic |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 1889.01 |
|---|---|
| Aliphatic_Index | 45.88235 |
| Aromaticity | 0.29412 |
| Average_Rotatable_Bonds | 3.29412 |
| Charge_at_pH_7 | -1.91106 |
| Isoelectric_Point | 4.10657 |
|---|---|
| Hydrogen_Bond_Acceptors | 24 |
| Hydrogen_Bond_Donors | 25 |
| Topological_Polar_Surface_Area | 731.54000 |
| X_logP_energy | -5.41240 |
Interaction Information
| Affinity | KD=0.6 uM |
|---|---|
| Affinity_Assay | Competition binding assay |
| PDB_ID | None |
| Type | Antagonist |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Anantin--a peptide antagonist of the atrial natriuretic factor (ANF). II. Determination of the primary sequence by NMR on the basis of proton assignments |
| Release_Year | 1991 |
| PMID | 1826288 |
| DOI | 10.7164/antibiotics.44.172 |