PPIRE19213

Target Protein Information
Protein_Name Adapter molecule crk
Protein_Sequence MAGNFDSEERSSWYWGRLSRQEAVALLQGQRHGVFLVRDSSTSPGDYVLSVSENSRVSHYIINSSGPRPPVPPSPAQPPPGVSPSRLRIGDQEFDSLPALLEFYKIHYLDTTTLIEPVSRSRQGSGVILRQEEAEYVRALFDFNGNDEEDLPFKKGDILRIRDKPEEQWWNAEDSEGKRGMIPVPYVEKYRPASASVSALIGGNQEGSHPQPLGGPEPGPYAQPSVNTPLPNLQNGPIYARVIQKRVPNAYDKTALALEVGELVKVTKINVSGQWEGECNGKRGHFPFTHVRLLDQQNPDEDFS
Organism_Source Homo sapiens
Functional_Classification SH3 domain containing proteins
Cellular_Localization Cytoplasm
Gene_Names CRK
UniProt_ID P46108
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name Sos peptide
Peptide_Sequence PPPVPPRRRR
Peptide_Length 10
Peptide_SMILES CC(C)[C@H](NC(=O)[C@@H]1CCCN1C(=O)[C@@H]1CCCN1C(=O)[C@@H]1CCCN1)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)O
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1227.48
Aliphatic_Index 29.00000
Aromaticity 0.00000
Average_Rotatable_Bonds 3.20000
Charge_at_pH_7 3.99797
Isoelectric_Point 12.97715
Hydrogen_Bond_Acceptors 15
Hydrogen_Bond_Donors 19
Topological_Polar_Surface_Area 523.67000
X_logP_energy -5.07992
Interaction Information
Affinity KD=200 uM
Affinity_Assay NMR chemical shift perturbation
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Role of electrostatic interactions in binding of peptides and intrinsically disordered proteins to their folded targets. 2. The model of encounter complex involving the double mutant of the c-Crk N-SH3 domain and peptide Sos.
Release_Year 2016
PMID 26910732
DOI 10.1021/acs.biochem.5b01283