PPIRE00346
Target Protein Information
| Protein_Name | LexA repressor |
|---|---|
| Protein_Sequence | MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEEHLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSLFKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGNKVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL |
| Organism_Source | Escherichia coli (strain K12) |
| Functional_Classification | Transcriptional regulator |
| Cellular_Localization | Nucleus |
| Gene_Names | lexA |
| UniProt_ID | P0A7C2 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | L2 Lariat peptide |
|---|---|
| Peptide_Sequence | RSWDLPGEY |
| Peptide_Length | 9 |
| Peptide_SMILES | CC(C)C[C@H](NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CCCNC(=N)N)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)O |
| Chemical_Modification | None |
| Cyclization_Method | S2<->E8; Main chain cyclization; lactone bond |
| Linear/Cyclic | Cyclic |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 1122.20 |
|---|---|
| Aliphatic_Index | 43.33333 |
| Aromaticity | 0.22222 |
| Average_Rotatable_Bonds | 3.55556 |
| Charge_at_pH_7 | -1.00065 |
| Isoelectric_Point | 4.18441 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 15 |
| Number_of_Hydrogen_Bond_Donors | 17 |
| Topological_Polar_Surface_Area | 480.08000 |
| X_logP_energy | -3.27223 |
Interaction Information
| Affinity | KD=6.3 nM |
|---|---|
| Affinity_Assay | Surface plasmon resonance |
| PDB_ID | None |
| Type | Agonist |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | A genetic screen for isolating lariat Peptide inhibitors of protein function. |
| Release_Year | 2009 |
| PMID | 19942138 |
| DOI | 10.1016/j.chembiol.2009.10.012 |