PPIRE01235

Target Protein Information
Protein_Name Regulator of Ty1 transposition protein 103
Protein_Sequence MPFSSEQFTTKLNTLEDSQESISSASKWLLLQYRDAPKVAEMWKEYMLRPSVNTRRKLLGLYLMNHVVQQAKGQKIIQFQDSFGKVAAEVLGRINQEFPRDLKKKLSRVVNILKERNIFSKQVVNDIERSLKTESSPVEALVLPQKLKDFAKDYEKLVKMHHNVCAMKMRFDKSSDELDPSSSVYEENFKTISKIGNMAKDIINESILKRESGIHKLQSTLDDEKRHLDEEQNMLSEIEFVLSAKDPSRLNKNVDEDNIIPTYEVGDGDDDDDDGDNDDDDDDDDDDKNYDDRSNDSNYGVTNISTTDKKNEVVEKTDSEHKNSTHNPSDNQFGMKRTHDMIGHDDANDIPEKKVHLDSKTSEDGTFNSEDGHYELDIEGHVGAQTDEGVENSGGVSSSIQDLLSKLAN
Organism_Source Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Functional_Classification transcription termination factors
Cellular_Localization nucleus
Gene_Names RTT103
UniProt_ID Q05543
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name Ser2P four-heptad CTD peptide
Peptide_Sequence YSPTSPSYSPTSPSYSPTSPSYSPTSPS
Peptide_Length 28
Peptide_SMILES C[C@@H](O)[C@H](NC(=O)[C@@H]1CCCN1C(=O)[C@H](CO)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CO)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)[C@H](CO)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CO)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)[C@H](CO)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CO)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CO)NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)[C@H](CO)NC(=O)[C@@H](N)Cc1ccc(O)cc1)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O)C(=O)N[C@@H](CO)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)O
Chemical_Modification S2=phosphoserine; S9=phosphoserine; S16=phosphoserine; S23=phosphoserine
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 2897.01
Aliphatic_Index 0.00000
Aromaticity 0.14286
Average_Rotatable_Bonds 2.53571
Charge_at_pH_7 -0.00542
Isoelectric_Point 6.07398
Number_of_Hydrogen_Bond_Acceptors 49
Number_of_Hydrogen_Bond_Donors 41
Topological_Polar_Surface_Area 1183.30000
X_logP_energy -21.23850
Interaction Information
Affinity KD=40 uM
Affinity_Assay fluorescence anisotropy
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Cooperative interaction of transcription termination factors with the RNA polymerase II C-terminal domain.
Release_Year 2010
PMID 20818393
DOI 10.1038/nsmb.1893