PPIRE01306
Target Protein Information
| Protein_Name | Cyclin-dependent kinases regulatory subunit 1 |
|---|---|
| Protein_Sequence | MSHKQIYYSDKYDDEEFEYRHVMLPKDIAKLVPKTHLMSESEWRNLGVQQSQGWVHYMIHEPEPHILLFRRPLPKKPKK |
| Organism_Source | Homo sapiens |
| Functional_Classification | E3 ubiquitin ligases |
| Cellular_Localization | nucleus |
| Gene_Names | CKS1B |
| UniProt_ID | P61024 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | p27Kip1(175-198)phosphopeptide |
|---|---|
| Peptide_Sequence | AGSVEQTPKK |
| Peptide_Length | 10 |
| Peptide_SMILES | CC(C)[C@H](NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](C)N)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O)[C@@H](C)O |
| Chemical_Modification | T7=phosphothreonine |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 1044.17 |
|---|---|
| Aliphatic_Index | 39.00000 |
| Aromaticity | 0.00000 |
| Average_Rotatable_Bonds | 3.50000 |
| Charge_at_pH_7 | 0.99917 |
| Isoelectric_Point | 9.53730 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 17 |
| Number_of_Hydrogen_Bond_Donors | 16 |
| Topological_Polar_Surface_Area | 489.32000 |
| X_logP_energy | -6.66360 |
Interaction Information
| Affinity | KD=9 uM |
|---|---|
| Affinity_Assay | Isothermal titration calorimetry |
| PDB_ID | None |
| Type | substrate |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Structural basis of the Cks1-dependent recognition of p27(Kip1)by the SCF(Skp2)ubiquitin ligase. |
| Release_Year | 2005 |
| PMID | 16209941 |
| DOI | 10.1016/j.molcel.2005.09.003 |