PPIRE01819

Target Protein Information
Protein_Name Ubiquitin carboxyl-terminal hydrolase isozyme L5
Protein_Sequence MTGNAGEWCLMESDPGVFTELIKGFGCRGAQVEEIWSLEPENFEKLKPVHGLIFLFKWQPGEEPAGSVVQDSRLDTIFFAKQVINNACATQAIVSVLLNCTHQDVHLGETLSEFKEFSQSFDAAMKGLALSNSDVIRQVHNSFARQQMFEFDTKTSAKEEDAFHFVSYVPVNGRLYELDGLREGPIDLGACNQDDWISAVRPVIEKRIQKYSEGEIRFNLMAIVSDRKMIYEQKIAELQRQLAEEEPMDTDQGNSMLSAIQSEVAKNQMLIEEEVQKLKRYKIENIRRKHNYLPFIMELLKTLAEHQQLIPLVEKAKEKQNAKKAQETK
Organism_Source Homo sapiens
Functional_Classification Enzyme
Cellular_Localization Cytoplasm
Gene_Names UCHL5
UniProt_ID Q9Y5K5
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name Ub1-17 cyclic peptide
Peptide_Sequence MQIFVKTLTGKTITLEV
Peptide_Length 17
Peptide_SMILES CC[C@H](C)[C@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H](N)CCSC)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@H](C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@H](C(=O)O)C(C)C)[C@@H](C)O)[C@@H](C)CC)[C@@H](C)O)[C@@H](C)O)[C@@H](C)O)C(C)C
Chemical_Modification None
Cyclization_Method M1<->V17, main chain cyclization, amide bond
Linear/Cyclic Cyclic
N-terminal_Modification Other
C-terminal_Modification other
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1922.35
Aliphatic_Index 125.88235
Aromaticity 0.05882
Average_Rotatable_Bonds 3.88235
Charge_at_pH_7 0.99917
Isoelectric_Point 9.53730
Number_of_Hydrogen_Bond_Acceptors 27
Number_of_Hydrogen_Bond_Donors 26
Topological_Polar_Surface_Area 742.27000
X_logP_energy -4.80980
Interaction Information
Affinity IC50=1.6 uM
Affinity_Assay Enzyme Inhibition Kinetics
PDB_ID None
Type Inhibitor
Structure
Reference Information
Document_Type Research Articles
Title Inhibiting UCH-L5: Rational Design of a Cyclic Ubiquitin-Based Peptide Inhibitor.
Release_Year 2022
PMID 35720124
DOI 10.3389/fmolb.2022.866467