PPIRE05056
Target Protein Information
| Protein_Name | Chymase |
|---|---|
| Protein_Sequence | MLLLPLPLLLFLLCSRAEAGEIIGGTECKPHSRPYMAYLEIVTSNGPSKFCGGFLIRRNFVLTAAHCAGRSITVTLGAHNITEEEDTWQKLEVIKQFRHPKYNTSTLHHDIMLLKLKEKASLTLAVGTLPFPSQFNFVPPGRMCRVAGWGRTGVLKPGSDTLQEVKLRLMDPQACSHFRDFDHNLQLCVGNPRKTKSAFKGDSGGPLLCAGVAQGIVSYGRSDAKPPAVFTRISHYRPWINQILQAN |
| Organism_Source | Homo sapiens |
| Functional_Classification | serine peptidase |
| Cellular_Localization | Extracellular |
| Gene_Names | CMA1 |
| UniProt_ID | P23946 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | Z-RETF-(OPh)2 |
|---|---|
| Peptide_Sequence | RETF |
| Peptide_Length | 4 |
| Peptide_SMILES | C[C@@H](O)[C@H](NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H](N)CCCNC(=N)N)C(=O)N[C@@H](Cc1ccccc1)C(=O)O |
| Chemical_Modification | None |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Benzoxycarbonyl |
| C-terminal_Modification | di-phenyl phosphonate |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 551.60 |
|---|---|
| Aliphatic_Index | 0.00000 |
| Aromaticity | 0.25000 |
| Average_Rotatable_Bonds | 4.25000 |
| Charge_at_pH_7 | -0.00024 |
| Isoelectric_Point | 6.41015 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 8 |
| Number_of_Hydrogen_Bond_Donors | 10 |
| Topological_Polar_Surface_Area | 270.05000 |
| X_logP_energy | -2.39573 |
Interaction Information
| Affinity | IC50=3.8 nM |
|---|---|
| Affinity_Assay | Enzyme Inhibition Kinetics |
| PDB_ID | None |
| Type | Inhibitor |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Albumin is a substrate of human chymase. Prediction by combinatorial peptide screening and development of a selective inhibitor based on the albumin cleavage site. |
| Release_Year | 2003 |
| PMID | 12815038 |
| DOI | 10.1074/jbc.M304087200 |