PPIRE05130
Target Protein Information
| Protein_Name | Cysteine protease ATG4B |
|---|---|
| Protein_Sequence | MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPAIGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFHCQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVELQPSHLACPDVLNLSLDSSDVERLERFFDSEDEDFEILSL |
| Organism_Source | Homo sapiens |
| Functional_Classification | cysteine proteases |
| Cellular_Localization | Cytoplasm |
| Gene_Names | ATG4B |
| UniProt_ID | Q9Y4P1 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | suc-TFG-ACC |
|---|---|
| Peptide_Sequence | TFGX |
| Peptide_Length | 4 |
| Peptide_SMILES | C[C@@H](O)[C@H](N)C(=O)N[C@@H](Cc1ccccc1)C(=O)NCC(=O)NCC(=O)O |
| Chemical_Modification | X4=7-aminocoumarin-4-yl-acetic acid amide |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Succinyl |
| C-terminal_Modification | coumarin amide |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 380.40 |
|---|---|
| Aliphatic_Index | 0.00000 |
| Aromaticity | 0.25000 |
| Average_Rotatable_Bonds | 2.50000 |
| Charge_at_pH_7 | -0.00202 |
| Isoelectric_Point | 6.10000 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 6 |
| Number_of_Hydrogen_Bond_Donors | 6 |
| Topological_Polar_Surface_Area | 170.85000 |
| X_logP_energy | -2.26100 |
Interaction Information
| Affinity | Km=400 uM |
|---|---|
| Affinity_Assay | Enzyme Inhibition Kinetics |
| PDB_ID | None |
| Type | Affinity ligand |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Development of fluorescent peptide substrates and assays for the key autophagy-initiating cysteine protease enzyme, ATG4B. |
| Release_Year | 2015 |
| PMID | 25979376 |
| DOI | 10.1016/j.bmc.2015.04.064 |