PPIRE05377
Target Protein Information
| Protein_Name | Cathepsin B |
|---|---|
| Protein_Sequence | MWQLWASLCCLLVLANARSRPSFHPLSDELVNYVNKRNTTWQAGHNFYNVDMSYLKRLCGTFLGGPKPPQRVMFTEDLKLPASFDAREQWPQCPTIKEIRDQGSCGSCWAFGAVEAISDRICIHTNAHVSVEVSAEDLLTCCGSMCGDGCNGGYPAEAWNFWTRKGLVSGGLYESHVGCRPYSIPPCEHHVNGSRPPCTGEGDTPKCSKICEPGYSPTYKQDKHYGYNSYSVSNSEKDIMAEIYKNGPVEGAFSVYSDFLLYKSGVYQHVTGEMMGGHAIRILGWGVENGTPYWLVANSWNTDWGDNGFFKILRGQDHCGIESEVVAGIPRTDQYWEKI |
| Organism_Source | Homo sapiens |
| Functional_Classification | cysteine protease |
| Cellular_Localization | Lysosome |
| Gene_Names | CTSB |
| UniProt_ID | P07858 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | Z-Arg-Arg-AMC |
|---|---|
| Peptide_Sequence | XRRX |
| Peptide_Length | 4 |
| Peptide_SMILES | N=C(N)NCCC[C@H](NC(=O)CN)C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)O |
| Chemical_Modification | X1=benzyloxycarbonyl (Z) X4=AMC group |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
|
|
|
Peptide Physicochemical
| Molecular_Weight | 444.49 |
|---|---|
| Aliphatic_Index | 0.00000 |
| Aromaticity | 0.00000 |
| Average_Rotatable_Bonds | 3.75000 |
| Charge_at_pH_7 | 1.99798 |
| Isoelectric_Point | 12.50011 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 7 |
| Number_of_Hydrogen_Bond_Donors | 11 |
| Topological_Polar_Surface_Area | 274.42000 |
| X_logP_energy | -4.35796 |
Interaction Information
| Affinity | Km=357 mM |
|---|---|
| Affinity_Assay | Enzyme Inhibition Kinetics |
| PDB_ID | None |
| Type | Affinity ligand |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Characterisation of Tc-cpl-1, a cathepsin L-like cysteine protease from Toxocara canis infective larvae |
| Release_Year | 1998 |
| PMID | 9657332 |
| DOI | 10.1016/s0166-6851(97)00245-4 |