PPIRE05727
Target Protein Information
| Protein_Name | Na(+)/H(+)exchange regulatory cofactor NHE-RF1 |
|---|---|
| Protein_Sequence | MSADAAAGAPLPRLCCLEKGPNGYGFHLHGEKGKLGQYIRLVEPGSPAEKAGLLAGDRLVEVNGENVEKETHQQVVSRIRAALNAVRLLVVDPETDEQLQKLGVQVREELLRAQEAPGQAEPPAAAEVQGAGNENEPREADKSHPEQRELRPRLCTMKKGPSGYGFNLHSDKSKPGQFIRSVDPDSPAEASGLRAQDRIVEVNGVCMEGKQHGDVVSAIRAGGDETKLLVVDRETDEFFKKCRVIPSQEHLNGPLPVPFTNGEIQKENSREALAEAALESPRPALVRSASSDTSEELNSQDSPPKQDSTAPSSTSSSDPILDFNISLAMAKERAHQKRSSKRAPQMDWSKKNELFSNL |
| Organism_Source | Homo sapiens |
| Functional_Classification | PDZ domain |
| Cellular_Localization | Cytoplasm |
| Gene_Names | NHERF1 |
| UniProt_ID | O14745 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | CFTR-C peptide |
|---|---|
| Peptide_Sequence | QDTRL |
| Peptide_Length | 5 |
| Peptide_SMILES | CC(C)C[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](NC(=O)[C@H](CC(=O)O)NC(=O)[C@@H](N)CCC(N)=O)[C@@H](C)O)C(=O)O |
| Chemical_Modification | None |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 631.69 |
|---|---|
| Aliphatic_Index | 78.00000 |
| Aromaticity | 0.00000 |
| Average_Rotatable_Bonds | 4.20000 |
| Charge_at_pH_7 | -0.00157 |
| Isoelectric_Point | 6.33872 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 10 |
| Number_of_Hydrogen_Bond_Donors | 12 |
| Topological_Polar_Surface_Area | 342.24000 |
| X_logP_energy | -4.23213 |
Interaction Information
| Affinity | KD=312 nM |
|---|---|
| Affinity_Assay | fluorescence binding assay |
| PDB_ID | None |
| Type | Affinity ligand |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Ligand-induced dynamic changes in extended PDZ domains from NHERF1. |
| Release_Year | 2013 |
| PMID | 23583913 |
| DOI | 10.1016/j.jmb.2013.04.001 |