PPIRE05743
Target Protein Information
| Protein_Name | Beta sliding clamp |
|---|---|
| Protein_Sequence | MKFTVEREHLLKPLQQVSGPLGGRPTLPILGNLLLQVADGTLSLTGTDLEMEMVARVALVQPHEPGATTVPARKFFDICRGLPEGAEIAVQLEGERMLVRSGRSRFSLSTLPAADFPNLDDWQSEVEFTLPQATMKRLIEATQFSMAHQDVRYYLNGMLFETEGEELRTVATDGHRLAVCSMPIGQSLPSHSVIVPRKGVIELMRMLDGGDNPLRVQIGSNNIRAHVGDFIFTSKLVDGRFPDYRRVLPKNPDKHLEAGCDLLKQAFARAAILSNEKFRGVRLYVSENQLKITANNPEQEEAEEILDVTYSGAEMEIGFNVSYVLDVLNALKCENVRMMLTDSVSSVQIEDAASQSAAYVVMPMRL |
| Organism_Source | Escherichia coli (strain K12) |
| Functional_Classification | processivity factors |
| Cellular_Localization | Cytoplasm |
| Gene_Names | dnaN |
| UniProt_ID | P0A988 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | AcQLGLF |
|---|---|
| Peptide_Sequence | QLGLF |
| Peptide_Length | 5 |
| Peptide_SMILES | CC(C)C[C@H](NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@@H](N)CCC(N)=O)C(=O)N[C@@H](Cc1ccccc1)C(=O)O |
| Chemical_Modification | None |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Acetyl |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 576.69 |
|---|---|
| Aliphatic_Index | 156.00000 |
| Aromaticity | 0.20000 |
| Average_Rotatable_Bonds | 3.60000 |
| Charge_at_pH_7 | -0.00202 |
| Isoelectric_Point | 6.10000 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 7 |
| Number_of_Hydrogen_Bond_Donors | 7 |
| Topological_Polar_Surface_Area | 222.81000 |
| X_logP_energy | -0.43070 |
Interaction Information
| Affinity | KD=4.9 uM |
|---|---|
| Affinity_Assay | Fluorescence Polarization |
| PDB_ID | None |
| Type | Inhibitor |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Structural and thermodynamic dissection of linear motif recognition by the E. coli sliding clamp. |
| Release_Year | 2013 |
| PMID | 24090409 |
| DOI | 10.1021/jm401118f |