PPIRE08312
Target Protein Information
| Protein_Name | Histone-lysine N-methyltransferase ASHH2 |
|---|---|
| Protein_Sequence | MDCKENGVGDASGCNIDANSLASNLAMNTNEDFYEKLSSRGQNLDSVSSLEIPQTASSVNHTIEGQRKCFTEIEQMGYGNSNSQEDAGNTDDDLYVCYNADDTQEQGVVSGELEQSQELICDTDLLVNCNKLDDGKESQDTNVSLVSIFSGSMQEKEAPQAKEDEGYGGTTLPIGGSGIDTESTFVNDAPEQFESLETTKHIKPDEVESDGISYRFDDGGKEGRNGPSSDLDTGSSDDISLSQSFSFPDSLLDSSVFGCSATESYLEDAIDIEGNGTIVVSPSLAITEMLNNDDGGLCSHDLNKITVTETINPDLKLVREDRLDTDLSVMNEKMLKNHVGDSSSESAVAALSMNNGMAADLRAENFSQSSPIDEKTLDMEANSPITDSSLIWNFPLNFGSGGIEVCNPENAVEPLRIVDDNGRIGGEVASASGSDFCEAGMSSSRRKARDGKQCKVVQTKTSARHLRKSSRKKQSERDIESIFKCSKQKRSSLLKTSRSSEWGLPSKTTEIFLQSNNIPYDGPPHHEPQRSQGNLNNGEHNRSSHNGNVEGSNRNIQASSGSCLRLKVKFGKSGGQNPLNITVSKVSGNSLPGNGIVKAGTCLELPGSAHFGEDKMQTVETKEDLVEKSNPVEKVSYLQSSDSMRDKKYNQDAGGLCRKVGGDVLDDDPHLSSIRMVEECERATGTQSLDAETSPDSEVINSVPDSIVNIEHKEGLHHGFFSTPEDVVKKNRVLEKEDELRASKSPSENGSHLIPNAKKAKHPKSKSNGTKKGKSKFSESAKDGRKNESHEGVEQRKSLNTSMGRDDSDYPEVGRIESHKTTGALLDADIGKTSATYGTISSDVTHGEMVVDVTIEDSYSTESAWVRCDDCFKWRRIPASVVGSIDESSRWICMNNSDKRFADCSKSQEMSNEEINEELGIGQDEADAYDCDAAKRGKEKEQKSKRLTGKQKACFKAIKTNQFLHRNRKSQTIDEIMVCHCKPSPDGRLGCGEECLNRMLNIECLQGTCPAGDLCSNQQFQKRKYVKFERFQSGKKGYGLRLLEDVREGQFLIEYVGEVLDMQSYETRQKEYAFKGQKHFYFMTLNGNEVIDAGAKGNLGRFINHSCEPNCRTEKWMVNGEICVGIFSMQDLKKGQELTFDYNYVRVFGAAAKKCYCGSSHCRGYIGGDPLNGDVIIQSDSDEEYPELVILDDDESGEGILGATSRTFTDDADEQMPQSFEKVNGYKDLAPDNTQTQSSVSVKLPEREIPPPLLQPTEVLKELSSGISITAVQQEVPAEKKTKSTSPTSSSLSRMSPGGTNSDKTTKHGSGEDKKILPRPRPRMKTSRSSESSKRDKGGIYPGVNKAQVIPVNKLQQQPIKSKGSEKVSPSIETFEGKLNELLDAVGGISKRRDSAKGYLKLLLLTAASRGTDEEGIYSNRDLSMILDALLKTKSKSVLVDIINKNGPFAGMESFKDSVLSFTEHDDYTVHNIARSFRDRWIPKHFRKPWRINREERSESMRSPINRRFRASQEPRYDHQSPRPAEPAASVTSSKAATPETASVSEGYSEPNSGLPETNGRKRKSRWDQPSKTKEQRIMTILSQQTDETNGNQDVQDDLPPGFSSPCTDVPDAITAQPQQKFLSRLPVSYGIPLSIVHQFGSPGKEDPTTWSVAPGMPFYPFPPLPPVSHGEFFAKRNVRACSSSMGNLTYSNEILPATPVTDSTAPTRKRELFSSDIGTTYFRQQKQSVPPWLRNNGGEKTANSPIPGNLTLEKKLNS |
| Organism_Source | Arabidopsis thaliana |
| Functional_Classification | histone methyltransferases |
| Cellular_Localization | Nucleus |
| Gene_Names | ASHH2 |
| UniProt_ID | Q2LAE1 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | H3K4me3 |
|---|---|
| Peptide_Sequence | ARTXQTARK |
| Peptide_Length | 9 |
| Peptide_SMILES | C[C@H](N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@H](C(=O)NCC(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCCN)C(=O)O)[C@@H](C)O)[C@@H](C)O |
| Chemical_Modification | X4=trimethyllysine |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 988.11 |
|---|---|
| Aliphatic_Index | 22.22222 |
| Aromaticity | 0.00000 |
| Average_Rotatable_Bonds | 3.77778 |
| Charge_at_pH_7 | 2.99768 |
| Isoelectric_Point | 12.51648 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 16 |
| Number_of_Hydrogen_Bond_Donors | 20 |
| Topological_Polar_Surface_Area | 529.49000 |
| X_logP_energy | -8.36946 |
Interaction Information
| Affinity | KD=18.9 uM |
|---|---|
| Affinity_Assay | Isothermal titration calorimetry |
| PDB_ID | None |
| Type | Affinity ligand |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Uncovering the mechanistic basis for specific recognition of monomethylated H3K4 by the CW domain of Arabidopsis histone methyltransferase SDG8. |
| Release_Year | 2018 |
| PMID | 29496997 |
| DOI | 10.1074/jbc.RA117.001390 |