PPIRE08617

Target Protein Information
Protein_Name Tumor susceptibility gene 101 protein
Protein_Sequence MAVSESQLKKMVSKYKYRDLTVRETVNVITLYKDLKPVLDSYVFNDGSSRELMNLTGTIPVPYRGNTYNIPICLWLLDTYPYNPPICFVKPTSSMTIKTGKHVDANGKIYLPYLHEWKHPQSDLLGLIQVMIVVFGDEPPVFSRPISASYPPYQATGPPNTSYMPGMPGGISPYPSGYPPNPSGYPGCPYPPGGPYPATTSSQYPSQPPVTTVGPSRDGTISEDTIRASLISAVSDKLRWRMKEEMDRAQAELNALKRTEEDLKKGHQKLEEMVTRLDQEVAEVDKNIELLKKKDEELSSALEKMENQSENNDIDEVIIPTAPLYKQILNLYAEENAIEDTIFYLGEALRRGVIDLDVFLKHVRLLSRKQFQLRALMQKARKTAGLSDLY
Organism_Source Homo sapiens
Functional_Classification ubiquitin E2 variant
Cellular_Localization Cytoplasm
Gene_Names TSG101
UniProt_ID Q99816
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name Gag PTAP P7A mutant
Peptide_Sequence PEATAPPEE
Peptide_Length 9
Peptide_SMILES C[C@H](NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H]1CCCN1)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N1CCC[C@H]1C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)O)[C@@H](C)O
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 939.97
Aliphatic_Index 22.22222
Aromaticity 0.00000
Average_Rotatable_Bonds 2.77778
Charge_at_pH_7 -2.99669
Isoelectric_Point 3.47280
Number_of_Hydrogen_Bond_Acceptors 14
Number_of_Hydrogen_Bond_Donors 12
Topological_Polar_Surface_Area 396.68000
X_logP_energy -4.27120
Interaction Information
Affinity KD=130 mM
Affinity_Assay Isothermal titration calorimetry
PDB_ID 3OBX
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Crystallographic and functional analysis of the ESCRT-I /HIV-1 Gag PTAP interaction.
Release_Year 2010
PMID 21070952
DOI 10.1016/j.str.2010.08.010