PPIRE09329
Target Protein Information
| Protein_Name | Serine endoprotease DegS |
|---|---|
| Protein_Sequence | MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETPASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDADQIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGNPYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTLSFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGIDQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVVVMRDDKQLTLQVTIQEYPATN |
| Organism_Source | Escherichia coli O157:H7 |
| Functional_Classification | serine proteases |
| Cellular_Localization | Extracellular |
| Gene_Names | degS |
| UniProt_ID | P0AEE4 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | OMP peptide |
|---|---|
| Peptide_Sequence | DNRDGNVYYF |
| Peptide_Length | 10 |
| Peptide_SMILES | CC(C)[C@H](NC(=O)[C@H](CC(N)=O)NC(=O)CNC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](N)CC(=O)O)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](Cc1ccccc1)C(=O)O |
| Chemical_Modification | None |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 1262.30 |
|---|---|
| Aliphatic_Index | 29.00000 |
| Aromaticity | 0.30000 |
| Average_Rotatable_Bonds | 3.80000 |
| Charge_at_pH_7 | -1.00283 |
| Isoelectric_Point | 4.10925 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 18 |
| Number_of_Hydrogen_Bond_Donors | 20 |
| Topological_Polar_Surface_Area | 588.36000 |
| X_logP_energy | -5.84833 |
Interaction Information
| Affinity | KD=4 mM |
|---|---|
| Affinity_Assay | fluorescence anisotropy |
| PDB_ID | 3GDS |
| Type | Allosteric modulator |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | A conserved activation cluster is required for allosteric communication in HtrA-family proteases. |
| Release_Year | 2015 |
| PMID | 25703375 |
| DOI | 10.1016/j.str.2015.01.012 |