PPIRE09506

Target Protein Information
Protein_Name Histone chaperone ASF1
Protein_Sequence MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSILVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEEELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGVDDEEEEDDEEEDDDEDDEDDEDDDQEDGEGEAEEAAEEEEEEEEKTEDNETNLEEEEEDIENSDGDEEEGEEEVGSVDKNEDGNDKKRRKIEGGSTDIESTPKDAARSTN
Organism_Source Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Functional_Classification histone chaperone
Cellular_Localization Nucleus
Gene_Names ASF1
UniProt_ID P32447
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name Rtt109419-428
Peptide_Sequence LAITMLKPRK
Peptide_Length 10
Peptide_SMILES CC[C@H](C)[C@H](NC(=O)[C@H](C)NC(=O)[C@@H](N)CC(C)C)C(=O)N[C@H](C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCCN)C(=O)O)[C@@H](C)O
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1170.52
Aliphatic_Index 127.00000
Aromaticity 0.00000
Average_Rotatable_Bonds 4.00000
Charge_at_pH_7 2.99739
Isoelectric_Point 11.82306
Number_of_Hydrogen_Bond_Acceptors 16
Number_of_Hydrogen_Bond_Donors 16
Topological_Polar_Surface_Area 450.60000
X_logP_energy -1.91893
Interaction Information
Affinity KD=44.1 uM
Affinity_Assay Isothermal titration calorimetry
PDB_ID 6F0Y
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Structural characterization of the Asf1-Rtt109 interaction and its role in histone acetylation.
Release_Year 2017
PMID 29300933
DOI 10.1093/nar/gkx1283