PPIRE09858

Target Protein Information
Protein_Name Serine endoprotease DegS
Protein_Sequence MFVKLLRSVAIGLIVGAILLVAMPSLRSLNPLSTPQFDSTDETPASYNLAVRRAAPAVVNVYNRGLNTNSHNQLEIRTLGSGVIMDQRGYIITNKHVINDADQIIVALQDGRVFEALLVGSDSLTDLAVLKINATGGLPTIPINARRVPHIGDVVLAIGNPYNLGQTITQGIISATGRIGLNPTGRQNFLQTDASINHGNSGGALVNSLGELMGINTLSFDKSNDGETPEGIGFAIPFQLATKIMDKLIRDGRVIRGYIGIGGREIAPLHAQGGGIDQLQGIVVNEVSPDGPAANAGIQVNDLIISVDNKPAISALETMDQVAEIRPGSVIPVVVMRDDKQLTLQVTIQEYPATN
Organism_Source Escherichia coli O157:H7
Functional_Classification serine proteases
Cellular_Localization Extracellular
Gene_Names degS
UniProt_ID P0AEE4
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name LKF peptide
Peptide_Sequence DNRLGLVLKF
Peptide_Length 10
Peptide_SMILES CC(C)C[C@H](NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H](N)CC(=O)O)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](Cc1ccccc1)C(=O)O)C(C)C
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1174.41
Aliphatic_Index 146.00000
Aromaticity 0.10000
Average_Rotatable_Bonds 4.00000
Charge_at_pH_7 0.99813
Isoelectric_Point 9.69502
Number_of_Hydrogen_Bond_Acceptors 15
Number_of_Hydrogen_Bond_Donors 17
Topological_Polar_Surface_Area 493.53000
X_logP_energy -2.82773
Interaction Information
Affinity KD=300 uM
Affinity_Assay Isothermal titration calorimetry
PDB_ID None
Type Allosteric modulator
Structure
Reference Information
Document_Type Research Articles
Title Regulation of the sigmaE stress response by DegS: how the PDZ domain keeps the protease inactive in the resting state and allows integration of different OMP-derived stress signals upon folding stress.
Release_Year 2007
PMID 17938245
DOI 10.1101/gad.445307