PPIRE09868
Target Protein Information
| Protein_Name | Stringent starvation protein B |
|---|---|
| Protein_Sequence | MDLSQLTPRRPYLLRAFYEWLLDNQLTPHLVVDVTLPGVQVPMEYARDGQIVLNIAPRAVGNLELANDEVRFNARFGGIPRQVSVPLAAVLAIYARENGAGTMFEPEAAYDEDTSIMNDEEASADNETVMSVIDGDKPDHDDDTHPDDEPPQPPRGGRPALRVVK |
| Organism_Source | Escherichia coli (strain K12) |
| Functional_Classification | Cholera toxin B subunit |
| Cellular_Localization | Cytoplasm |
| Gene_Names | sspB |
| UniProt_ID | P0AFZ3 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | SsrA tag |
|---|---|
| Peptide_Sequence | AANDENYALAA |
| Peptide_Length | 11 |
| Peptide_SMILES | CC(C)C[C@H](NC(=O)[C@H](C)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](C)N)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)O |
| Chemical_Modification | None |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 1122.16 |
|---|---|
| Aliphatic_Index | 80.90909 |
| Aromaticity | 0.09091 |
| Average_Rotatable_Bonds | 3.09091 |
| Charge_at_pH_7 | -2.00064 |
| Isoelectric_Point | 3.55007 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 17 |
| Number_of_Hydrogen_Bond_Donors | 17 |
| Topological_Polar_Surface_Area | 535.33000 |
| X_logP_energy | -6.56950 |
Interaction Information
| Affinity | KD=6.16 uM |
|---|---|
| Affinity_Assay | Isothermal titration calorimetry |
| PDB_ID | None |
| Type | Affinity ligand |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Structural basis of degradation signal recognition by SspB, a specificity-enhancing factor for the ClpXP proteolytic machine. |
| Release_Year | 2003 |
| PMID | 12887894 |
| DOI | 10.1016/s1097-2765(03)00271-5 |