PPIRE09930

Target Protein Information
Protein_Name SH2/SH3 adapter protein NCK1
Protein_Sequence MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS
Organism_Source Homo sapiens
Functional_Classification SH2 domain-containing adapter proteins
Cellular_Localization Cytoplasm
Gene_Names NCK1
UniProt_ID P16333
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name EphrinB1/2
Peptide_Sequence CPHpYEKVSGD
Peptide_Length 11
Peptide_SMILES CC(C)[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@@H]1CCCN1C(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CS)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CC(=O)O)C(=O)O
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1231.35
Aliphatic_Index 26.36364
Aromaticity 0.09091
Average_Rotatable_Bonds 3.18182
Charge_at_pH_7 -0.97201
Isoelectric_Point 5.45176
Number_of_Hydrogen_Bond_Acceptors 19
Number_of_Hydrogen_Bond_Donors 17
Topological_Polar_Surface_Area 506.50000
X_logP_energy -5.14920
Interaction Information
Affinity KD=22 uM
Affinity_Assay Surface plasmon resonance
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title The phosphotyrosine peptide binding specificity of Nck1 and Nck2 Src homology 2 domains.
Release_Year 2006
PMID 16636066
DOI 10.1074/jbc.M512917200