PPIRE09943

Target Protein Information
Protein_Name Cytoplasmic protein NCK2
Protein_Sequence MTEEVIVIAKWDYTAQQDQELDIKKNERLWLLDDSKTWWRVRNAANRTGYVPSNYVERKNSLKKGSLVKNLKDTLGLGKTRRKTSARDASPTPSTDAEYPANGSGADRIYDLNIPAFVKFAYVAEREDELSLVKGSRVTVMEKCSDGWWRGSYNGQIGWFPSNYVLEEVDEAAAESPSFLSLRKGASLSNGQGSRVLHVVQTLYPFSSVTEEELNFEKGETMEVIEKPENDPEWWKCKNARGQVGLVPKNYVVVLSDGPALHPAHAPQISYTGPSSSGRFAGREWYYGNVTRHQAECALNERGVEGDFLIRDSESSPSDFSVSLKASGKNKHFKVQLVDNVYCIGQRRFHTMDELVEHYKKAPIFTSEHGEKLYLVRALQ
Organism_Source Homo sapiens
Functional_Classification SH2 domain-containing adapter proteins
Cellular_Localization Cytoplasm
Gene_Names NCK2
UniProt_ID O43639
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name A36R
Peptide_Sequence EHIpYDSVAGS
Peptide_Length 11
Peptide_SMILES CC[C@H](C)[C@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@@H](N)CCC(=O)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CO)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)NCC(=O)N[C@@H](CO)C(=O)O)C(C)C
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1174.23
Aliphatic_Index 70.90909
Aromaticity 0.09091
Average_Rotatable_Bonds 3.09091
Charge_at_pH_7 -1.90974
Isoelectric_Point 4.18115
Number_of_Hydrogen_Bond_Acceptors 18
Number_of_Hydrogen_Bond_Donors 17
Topological_Polar_Surface_Area 509.50000
X_logP_energy -5.65590
Interaction Information
Affinity KD=150 uM
Affinity_Assay Surface plasmon resonance
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title The phosphotyrosine peptide binding specificity of Nck1 and Nck2 Src homology 2 domains.
Release_Year 2006
PMID 16636066
DOI 10.1074/jbc.M512917200