PPIRE10087

Target Protein Information
Protein_Name Speckle-type POZ protein
Protein_Sequence MSRVPSPPPPAEMSSGPVAESWCYTQIKVVKFSYMWTINNFSFCREEMGEVIKSSTFSSGANDKLKWCLRVNPKGLDEESKDYLSLYLLLVSCPKSEVRAKFKFSILNAKGEETKAMESQRAYRFVQGKDWGFKKFIRRDFLLDEANGLLPDDKLTLFCEVSVVQDSVNISGQNTMNMVKVPECRLADELGGLWENSRFTDCCLCVAGQEFQAHKAILAARSPVFSAMFEHEMEESKKNRVEINDVEPEVFKEMMCFIYTGKAPNLDKMADDLLAAADKYALERLKVMCEDALCSNLSVENAAEILILADLHSADQLKTQAVDFINYHASDVLETSGWKSMVVSHPHLVAEAYRSLASAQCPFLGPPRKRLKQS
Organism_Source Homo sapiens
Functional_Classification ubiquitin-protein ligases
Cellular_Localization Nucleus
Gene_Names SPOP
UniProt_ID O43791
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name Pdx1 SPOP-binding peptide(219-233)P-Ser231
Peptide_Sequence PEQDCAVTSGE
Peptide_Length 11
Peptide_SMILES CC(C)[C@H](NC(=O)[C@H](C)NC(=O)[C@H](CS)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H]1CCCN1)C(=O)N[C@H](C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](CCC(=O)O)C(=O)O)[C@@H](C)O
Chemical_Modification S231=phosphoserine
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1135.17
Aliphatic_Index 35.45455
Aromaticity 0.00000
Average_Rotatable_Bonds 3.27273
Charge_at_pH_7 -3.05999
Isoelectric_Point 3.42471
Number_of_Hydrogen_Bond_Acceptors 19
Number_of_Hydrogen_Bond_Donors 19
Topological_Polar_Surface_Area 535.78000
X_logP_energy -8.24900
Interaction Information
Affinity KD=715.8 uM
Affinity_Assay Isothermal titration calorimetry
PDB_ID 6F8F
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title The Structure of the SPOP-Pdx1 Interface Reveals Insights into the Phosphorylation-Dependent Binding Regulation.
Release_Year 2018
PMID 30449689
DOI 10.1016/j.str.2018.10.005