PPIRE10104

Target Protein Information
Protein_Name 14-3-3 protein zeta/delta
Protein_Sequence MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN
Organism_Source Homo sapiens
Functional_Classification adapter proteins
Cellular_Localization Cytoplasm
Gene_Names YWHAZ
UniProt_ID P63104
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name C-RAF Ser259 phosphopeptide(255-264)
Peptide_Sequence QRSTpSTPNVH
Peptide_Length 11
Peptide_SMILES CC(C)[C@H](NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](N)CCC(N)=O)[C@@H](C)O)[C@@H](C)O)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)O
Chemical_Modification S5=phosphoserine
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1223.31
Aliphatic_Index 26.36364
Aromaticity 0.00000
Average_Rotatable_Bonds 3.18182
Charge_at_pH_7 1.08889
Isoelectric_Point 10.55176
Number_of_Hydrogen_Bond_Acceptors 20
Number_of_Hydrogen_Bond_Donors 20
Topological_Polar_Surface_Area 594.42000
X_logP_energy -9.96913
Interaction Information
Affinity KD=7.5 uM
Affinity_Assay Isothermal titration calorimetry
PDB_ID 3NKX
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Impaired binding of 14-3-3 to C-RAF in Noonan syndrome suggests new approaches in diseases with increased Ras signaling.
Release_Year 2010
PMID 20679480
DOI 10.1128/MCB.01636-09