PPIRE10145

Target Protein Information
Protein_Name 14-3-3 protein zeta/delta
Protein_Sequence MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN
Organism_Source Homo sapiens
Functional_Classification scaffolding protein
Cellular_Localization Cytoplasm
Gene_Names YWHAZ
UniProt_ID P63104
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name FOXO1_pS22_pT24_peptide
Peptide_Sequence RPRSCTWPLPR
Peptide_Length 11
Peptide_SMILES CC(C)C[C@H](NC(=O)[C@@H]1CCCN1C(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@@H](NC(=O)[C@H](CS)NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CCCNC(=N)N)[C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(=N)N)C(=O)O
Chemical_Modification S4=phosphoserine; T6=phosphothreonine
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1368.62
Aliphatic_Index 35.45455
Aromaticity 0.09091
Average_Rotatable_Bonds 3.36364
Charge_at_pH_7 2.93600
Isoelectric_Point 12.19926
Number_of_Hydrogen_Bond_Acceptors 18
Number_of_Hydrogen_Bond_Donors 22
Topological_Polar_Surface_Area 569.90000
X_logP_energy -5.37229
Interaction Information
Affinity KD=12.9 uM
Affinity_Assay fluorescence anisotropy
PDB_ID None
Type Inhibitor
Structure
Reference Information
Document_Type Research Articles
Title AMPK and AKT protein kinases hierarchically phosphorylate the N-terminus of the FOXO1 transcription factor, modulating interactions with 14-3-3 proteins.
Release_Year 2019
PMID 31308176
DOI 10.1074/jbc.RA119.008649