PPIRE10192
Target Protein Information
| Protein_Name | E3 ubiquitin-protein ligase Nedd-4 |
|---|---|
| Protein_Sequence | MSARSSGLVAAAALPVPSSSSSVAGGDVPRPPPRRRAASVAGQQQTRQEFGNGYTPRRSLAAVNDSGDSCHLRIVVLTGQSLAKKDIFGASDPYVRIDLNTINGDINIDSVLTKTKKKTLNPTWNEEFIFRVKPSEHKLVFQVFDENRLTRDDFLGMVELTLVNLPTEQEGRTIGEQSYTLRPRRSVGAKSRIKGTLRIYHAFIRETREQSEPSSGNSDGEWEHVEATNAGETSAQPHPFPTGGHDALPAGWEERQDANGRTYYVNHTARTTQWDRPTVLNSHSSQSTDDQLASDFQRRFHISVDDTESGRSADSISHNSIEDNNNAAGLAYTPKTAATSSAPPNTPTNNNGILAQIAMQYRAEEDQDPTVDHTSFVYNSLRHPVAHRQPEISATSLQNDLRPVREAPGVPDIAITNPFTRRAAGNMAGGAGWQQERRRQQMQLHIQQHQQRQQQQQQNRILLDVDHRQQEPQHRGQRHQQQHRPSNEDTDHTDSHNPSDISAPSTRRNSEEDNAAVPPMEQNTGGEEEPLPPRWSMQVAPNGRTFFIDHASRRTTWIDPRNGRASPMPNQTRRVEDDLGPLPEGWEERVHTDGRVFYIDHNTRTTQWEDPRLSNPNIAGQAVPYSRDYKQKYEYFKSHIRKPTNVPNKFEIRIRRTSILEDSYRIISSVTKTDLLKTKLWVEFEGETGLDYGGLAREWFYLLSKEMFNPYYGLFEYSAMDNYTLQINNGSGLCNEEHLSYFKFIGRIAGMAVYHGKLLDAFFIRPFYKMMLQKPIDLKDMESVDTEYYNSLMWIKENDPRILELTFCLDEDVFGQKSQHELKPGGANIDVTNENKDEYIKLVIEWRFVARVKEQMSSFLDGFGSIIPLNLIKIFDEHELELLMCGIQNIDVKDWRENTLYKGDYHMNHIIIQWFWRAVLSFSNEMRSRLLQFVTGTSRVPMNGFKELYGSNGPQMFTIEKWGTPNNFPRAHTCFNRLDLPPYEGYLQLKDKLIKAIEGSQGFAGVD |
| Organism_Source | Drosophila melanogaster |
| Functional_Classification | ubiquitin ligases |
| Cellular_Localization | Cytoplasm |
| Gene_Names | Nedd4 |
| UniProt_ID | Q9VVI3 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | Comm LPSY L236A peptide |
|---|---|
| Peptide_Sequence | TGLPSYDEAAH |
| Peptide_Length | 11 |
| Peptide_SMILES | CC(C)C[C@H](NC(=O)CNC(=O)[C@@H](N)[C@@H](C)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)O |
| Chemical_Modification | None |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 1160.21 |
|---|---|
| Aliphatic_Index | 53.63636 |
| Aromaticity | 0.09091 |
| Average_Rotatable_Bonds | 3.00000 |
| Charge_at_pH_7 | -1.90974 |
| Isoelectric_Point | 4.18115 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 18 |
| Number_of_Hydrogen_Bond_Donors | 17 |
| Topological_Polar_Surface_Area | 509.50000 |
| X_logP_energy | -5.90350 |
Interaction Information
| Affinity | KD=8 mM |
|---|---|
| Affinity_Assay | intrinsic tryptophan fluorescence |
| PDB_ID | None |
| Type | Affinity ligand |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Structural determinants for high-affinity binding in a Nedd4 WW3* domain-Comm PY motif complex. |
| Release_Year | 2006 |
| PMID | 16531238 |
| DOI | 10.1016/j.str.2005.11.018 |