PPIRE10239
Target Protein Information
| Protein_Name | mRNA-capping enzyme |
|---|---|
| Protein_Sequence | MAYNKIPPRWLNCPRRGQPVAGRFLPLKTMLGPRYDSQVAEENRFHPSMLSNYLKSLKVKMSLLVDLTNTSRFYDRNDIEKEGIKYIKLQCKGHGECPTTENTETFIRLCERFNERSPPELIGVHCTHGFNRTGFLICAFLVEKMDWSIEAAVATFAQARPPGIYKGDYLKELFRRYGDIEEAPPPPVLPDWCFEDEDEEDEDEDGKKDSEPGSSASFSKRRKERLKLGAIFLEGITVKGVTQVTTQPKLGEVQQKCHQFCGWEGSGFPGAQPVSMDKQNIRLLEQKPYKVSWKADGTRYMMLIDGTNEVFMIDRDNSVFHVSNLEFPFRKDLRMHLSNTLLDGEMIIDKVNGQAVPRYLIYDIIKFNAQPVGDCDFNIRLQCIEREIISPRHEKMKTGLIDKTQEPFSVRPKQFFDINISRKLLEGNFAKEVSHEMDGLIFQPIGKYKPGRCDDILKWKPPSLNSVDFRLKITRMGGEGLLPQNVGLLYVGGYERPFAQIKVTKELKQYDNKIIECKFENNSWVFMRQRIDKSFPNAYNTAMAVCNSISNPVTKEMLFEFIDRCAAAAQGQKRKYPLDPDTELMPPPPPKRLHRPT |
| Organism_Source | Mus musculus |
| Functional_Classification | nucleotidyltransferases |
| Cellular_Localization | Nucleus |
| Gene_Names | Rngtt |
| UniProt_ID | O55236 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | CTD peptide |
|---|---|
| Peptide_Sequence | XPTXPSYXPTX |
| Peptide_Length | 11 |
| Peptide_SMILES | C[C@@H](O)[C@H](NC(=O)[C@@H]1CCCN1C(=O)CN)C(=O)NCC(=O)N1CCC[C@H]1C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)NCC(=O)N1CCC[C@H]1C(=O)N[C@H](C(=O)NCC(=O)O)[C@@H](C)O |
| Chemical_Modification | X1=phospho-serine; X4=phospho-serine; X8=phospho-serine; X11=phospho-serine |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 990.04 |
|---|---|
| Aliphatic_Index | 0.00000 |
| Aromaticity | 0.09091 |
| Average_Rotatable_Bonds | 2.09091 |
| Charge_at_pH_7 | -0.00287 |
| Isoelectric_Point | 6.09320 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 16 |
| Number_of_Hydrogen_Bond_Donors | 13 |
| Topological_Polar_Surface_Area | 408.87000 |
| X_logP_energy | -7.00730 |
Interaction Information
| Affinity | Ki=160 uM |
|---|---|
| Affinity_Assay | Fluorescence Polarization |
| PDB_ID | 3RTX |
| Type | Affinity ligand |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Structural insights to how mammalian capping enzyme reads the CTD code. |
| Release_Year | 2011 |
| PMID | 21683636 |
| DOI | 10.1016/j.molcel.2011.06.001 |