PPIRE11215

Target Protein Information
Protein_Name SH2/SH3 adapter protein NCK1
Protein_Sequence MAEEVVVVAKFDYVAQQEQELDIKKNERLWLLDDSKSWWRVRNSMNKTGFVPSNYVERKNSARKASIVKNLKDTLGIGKVKRKPSVPDSASPADDSFVDPGERLYDLNMPAYVKFNYMAEREDELSLIKGTKVIVMEKCSDGWWRGSYNGQVGWFPSNYVTEEGDSPLGDHVGSLSEKLAAVVNNLNTGQVLHVVQALYPFSSSNDEELNFEKGDVMDVIEKPENDPEWWKCRKINGMVGLVPKNYVTVMQNNPLTSGLEPSPPQCDYIRPSLTGKFAGNPWYYGKVTRHQAEMALNERGHEGDFLIRDSESSPNDFSVSLKAQGKNKHFKVQLKETVYCIGQRKFSTMEELVEHYKKAPIFTSEQGEKLYLVKHLS
Organism_Source Homo sapiens
Functional_Classification SH2 domain-containing adapter proteins
Cellular_Localization Cytoplasm
Gene_Names NCK1
UniProt_ID P16333
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name PDGFR1009
Peptide_Sequence SSVLpYTAVQPNE
Peptide_Length 13
Peptide_SMILES CC(C)C[C@H](NC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@@H](N)CO)C(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)N[C@H](C(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCC(=O)O)C(=O)O)C(C)C)[C@@H](C)O
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1404.54
Aliphatic_Index 82.30769
Aromaticity 0.07692
Average_Rotatable_Bonds 3.07692
Charge_at_pH_7 -1.00109
Isoelectric_Point 3.84998
Number_of_Hydrogen_Bond_Acceptors 21
Number_of_Hydrogen_Bond_Donors 19
Topological_Polar_Surface_Area 599.34000
X_logP_energy -7.07430
Interaction Information
Affinity KD=27 uM
Affinity_Assay Surface plasmon resonance
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title The phosphotyrosine peptide binding specificity of Nck1 and Nck2 Src homology 2 domains.
Release_Year 2006
PMID 16636066
DOI 10.1074/jbc.M512917200