PPIRE11295
Target Protein Information
| Protein_Name | Urokinase-type plasminogen activator |
|---|---|
| Protein_Sequence | MKVWLASLFLCALVVKNSEGGSVLGAPDESNCGCQNGGVCVSYKYFSRIRRCSCPRKFQGEHCEIDASKTCYHGNGDSYRGKANTDTKGRPCLAWNAPAVLQKPYNAHRPDAISLGLGKHNYCRNPDNQKRPWCYVQIGLRQFVQECMVHDCSLSKKPSSSVDQQGFQCGQKALRPRFKIVGGEFTEVENQPWFAAIYQKNKGGSPPSFKCGGSLISPCWVASAAHCFIQLPKKENYVVYLGQSKESSYNPGEMKFEVEQLILHEYYREDSLAYHNDIALLKIRTSTGQCAQPSRSIQTICLPPRFTDAPFGSDCEITGFGKESESDYLYPKNLKMSVVKLVSHEQCMQPHYYGSEINYKMLCAADPEWKTDSCKGDSGGPLICNIEGRPTLSGIVSWGRGCAEKNKPGVYTRVSHFLDWIQSHIGEEKGLAF |
| Organism_Source | Mus musculus |
| Functional_Classification | serine protease |
| Cellular_Localization | Extracellular |
| Gene_Names | Plau |
| UniProt_ID | P06869 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | mupain-1-16-IG |
|---|---|
| Peptide_Sequence | CPAYSYIGC |
| Peptide_Length | 9 |
| Peptide_SMILES | CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](CO)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)[C@H](C)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](N)CS)C(=O)NCC(=O)N[C@@H](CS)C(=O)O |
| Chemical_Modification | X6=L-3-(N-amidino-4-piperidyl)alanine |
| Cyclization_Method | Side chain-side chain cyclization; C1<->C10; disulfide bond |
| Linear/Cyclic | Cyclic |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 976.13 |
|---|---|
| Aliphatic_Index | 54.44444 |
| Aromaticity | 0.22222 |
| Average_Rotatable_Bonds | 2.77778 |
| Charge_at_pH_7 | -0.12767 |
| Isoelectric_Point | 5.82212 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 15 |
| Number_of_Hydrogen_Bond_Donors | 14 |
| Topological_Polar_Surface_Area | 348.02000 |
| X_logP_energy | -2.77120 |
Interaction Information
| Affinity | Ki=0.01 uM |
|---|---|
| Affinity_Assay | Enzyme Inhibition Kinetics |
| PDB_ID | None |
| Type | Inhibitor |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Selection of High-Affinity Peptidic Serine Protease Inhibitors with Increased Binding Entropy from a Back-Flip Library of Peptide-Protease Fusions. |
| Release_Year | 2015 |
| PMID | 26281711 |
| DOI | 10.1016/j.jmb.2015.08.005 |