PPIRE11451
Target Protein Information
| Protein_Name | Polyadenylate-binding protein |
|---|---|
| Protein_Sequence | MAAAVQEAAAPVAHQPQMDKPMQIASIYVGDLDATINEPQLVELFKPFGTILNVRVCRDIITQRSLGYGYVNFDNHDSAEKAIESMNFKRVGDKCVRLMWQQRDPALRYSGNGNVFVKNLEKDVDSKSLHDIFTKFGSILSCKVMQDEEGKSRGYGFVHFKDETSAKDAIVKMNGAADHASEDKKALYVANFIRRNARLAALVANFTNVYIKQVLPTVNKDVIEKFFAKFGGITSAAACKDKSGRVFAFCNFEKHDDAVKAVEAMHDHHIDGITAPGEKLYVQRAQPRSERLIALRQKYMQHQALGNNLYVRNFDPEFTGADLLELFKEYGEVKSCRVMVSESGVSRGFGFVSFSNADEANAALREMNGRMLNGKPLIVNIAQRRDQRYTMLRLQFQQRLQMMMRQMHQPMPFVGSQGRPMRGRGGRQQLGGRAQGHPMPMPSPQQPQAPAQPQGFATPSAVGFVQATPKHSPGDVPETPPLPPITPQELESMSPQEQRAALGDRLFLKVYEIAPELAPKITGMFLEMKPKEAYELLNDQKRLEERVTEALCVLKAHQTA |
| Organism_Source | Leishmania major |
| Functional_Classification | poly(A)-binding proteins |
| Cellular_Localization | Cytoplasm |
| Gene_Names | PABP1 |
| UniProt_ID | E9AFX7 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | PAM2-motif FITC-labelled peptide N143A |
|---|---|
| Peptide_Sequence | HHMNPAATEFMPGR |
| Peptide_Length | 14 |
| Peptide_SMILES | CSCC[C@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@@H](N)Cc1c[nH]cn1)C(=O)N[C@@H](CC(N)=O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](C)C(=O)N[C@@H](C)C(=O)N[C@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCSC)C(=O)N1CCC[C@H]1C(=O)NCC(=O)N[C@@H](CCCNC(=N)N)C(=O)O)[C@@H](C)O |
| Chemical_Modification | FITC attached via 6-aminohexanoic acid at residue 1 |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Fitc-6-Aminohexanoic Acid |
| C-terminal_Modification | amide |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 1595.81 |
|---|---|
| Aliphatic_Index | 14.28571 |
| Aromaticity | 0.07143 |
| Average_Rotatable_Bonds | 3.35714 |
| Charge_at_pH_7 | 0.18157 |
| Isoelectric_Point | 7.71482 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 23 |
| Number_of_Hydrogen_Bond_Donors | 21 |
| Topological_Polar_Surface_Area | 643.92000 |
| X_logP_energy | -6.55093 |
Interaction Information
| Affinity | KD=69.2 nM |
|---|---|
| Affinity_Assay | MicroScale Thermophoresis |
| PDB_ID | 6H7B |
| Type | Affinity ligand |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | The Leishmania PABP1-eIF4E4 interface: a novel 5'-3' interaction architecture for trans-spliced mRNAs. |
| Release_Year | 2018 |
| PMID | 30476241 |
| DOI | 10.1093/nar/gky1187 |