PPIRE11938

Target Protein Information
Protein_Name 14-3-3 protein gamma
Protein_Sequence MVDREQLVQKARLAEQAERYDDMAAAMKNVTELNEPLSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQYESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQPTHPIRLGLALNYSVFYYEIQNAPEQACHLAKTAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDDDGGEGNN
Organism_Source Homo sapiens
Functional_Classification scaffold proteins
Cellular_Localization Cytoplasm
Gene_Names YWHAG
UniProt_ID P61981
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name TMR-pS259-Raf
Peptide_Sequence LSQRQRSTXTPNVHM
Peptide_Length 15
Peptide_SMILES CSCC[C@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@@H](NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@@H](NC(=O)CNC(=O)[C@@H](NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@@H](N)CC(C)C)[C@@H](C)O)[C@@H](C)O)C(C)C)C(=O)O
Chemical_Modification X9=phosphoserine
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Tmr
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1711.92
Aliphatic_Index 45.33333
Aromaticity 0.00000
Average_Rotatable_Bonds 3.73333
Charge_at_pH_7 2.08889
Isoelectric_Point 12.50011
Number_of_Hydrogen_Bond_Acceptors 27
Number_of_Hydrogen_Bond_Donors 29
Topological_Polar_Surface_Area 824.60000
X_logP_energy -12.57676
Interaction Information
Affinity KD=0.41 uM
Affinity_Assay Fluorescence Polarization
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Fluorescence polarization assay to quantify protein-protein interactions in an HTS format.
Release_Year 2015
PMID 25859974
DOI 10.1007/978-1-4939-2425-7_35