PPIRE11957
Target Protein Information
| Protein_Name | Neuronal calcium sensor 1 |
|---|---|
| Protein_Sequence | MGKSNSKLKPEVVEELTRKTYFTEKEVQQWYKGFIKDCPSGQLDAAGFQKIYKQFFPFGDPTKFATFVFNVFDENKDGRIEFSEFIQALSVTSRGTLDEKLRWAFKLYDLDNDGYITRNEMLDIVDAIYQMVGNTVELPEEENTPEKRVDRIFAMMDKNADGKLTLQEFQEGSKADPSIVQALSLYDGLV |
| Organism_Source | Rattus norvegicus |
| Functional_Classification | calcium sensor proteins |
| Cellular_Localization | Cytoplasm |
| Gene_Names | Ncs1 |
| UniProt_ID | P62168 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | D2R peptide |
|---|---|
| Peptide_Sequence | NIEFRKAFLKILHSR |
| Peptide_Length | 15 |
| Peptide_SMILES | CC[C@H](C)[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](C)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H](NC(=O)[C@@H](N)CC(N)=O)[C@@H](C)CC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCCNC(=N)N)C(=O)O |
| Chemical_Modification | None |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 1872.25 |
|---|---|
| Aliphatic_Index | 110.66667 |
| Aromaticity | 0.13333 |
| Average_Rotatable_Bonds | 4.33333 |
| Charge_at_pH_7 | 3.09007 |
| Isoelectric_Point | 11.65090 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 24 |
| Number_of_Hydrogen_Bond_Donors | 28 |
| Topological_Polar_Surface_Area | 775.86000 |
| X_logP_energy | -4.64196 |
Interaction Information
| Affinity | KD=40 uM |
|---|---|
| Affinity_Assay | Isothermal titration calorimetry |
| PDB_ID | 5AER |
| Type | Affinity ligand |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Neuronal Calcium Sensor-1 Binds the D2 Dopamine Receptor and G-protein-coupled Receptor Kinase 1 (GRK1)Peptides Using Different Modes of Interactions. |
| Release_Year | 2015 |
| PMID | 25979333 |
| DOI | 10.1074/jbc.M114.627059 |