PPIRE11965

Target Protein Information
Protein_Name Importin subunit alpha
Protein_Sequence MADRYIPEHRRTQFKAKSAFKPDELRRRREEQQVEIRKAKREENLAKRRGIGAGDSRPGASLGAAPDSDDENPPTESQLSEDLPKMVEGVFSSEIDKQIQATTKFRKLLSKERNPPIEEVIKTGVVGRFVEFLRSPHTLVQFEAAWALTNIASGSATQTQVVIEAGAVPIFVELLGSPEPDVREQAVWALGNIAGDSPQCRDYVLSCGALRPLLTLLGDSRKLSMLRNATWTLSNFCRGKTPQPDWNTIAPALPVLAKLVYSLDDEVLIDACWAISYLSDGSNDKIQAVIEAGIPRRLVELLMHASTSVQTPALRSVGNIVTGDDVQTQVIINCGALPCLLSLLSSNKDGIRKEACWTISNITAGNSAQIQSVIDANIIPPLIHLLSHADLKTRKEACWAISNATSGGLQKPDQIRYLVAQGCIKPLCDLLACPDNKIIQVALDGLENILKVGELDKNAAGDGPDSINRYALFIEECGGMEKIHDCQTNANEEIYMKAYNIIEKYFSDEDEAGDEAMGAQQQFGFGASGGAQQGGFNFGANGTESMDM
Organism_Source Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987)
Functional_Classification importin
Cellular_Localization Cytoplasm
Gene_Names None
UniProt_ID Q1K6K3
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name SV40 NLS peptide
Peptide_Sequence PPKKKRKV
Peptide_Length 8
Peptide_SMILES CC(C)[C@H](NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCCN)NC(=O)[C@@H]1CCCN1C(=O)[C@@H]1CCCN1)C(=O)O
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 980.27
Aliphatic_Index 36.25000
Aromaticity 0.00000
Average_Rotatable_Bonds 4.37500
Charge_at_pH_7 4.99680
Isoelectric_Point 11.99738
Number_of_Hydrogen_Bond_Acceptors 14
Number_of_Hydrogen_Bond_Donors 15
Topological_Polar_Surface_Area 410.22000
X_logP_energy -2.84193
Interaction Information
Affinity KD=1.69 uM
Affinity_Assay Isothermal titration calorimetry
PDB_ID 4RXH
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Structure of Importin-Alpha from a Filamentous Fungus in Complex with a Classical Nuclear Localization Signal.
Release_Year 2015
PMID 26091498
DOI 10.1371/journal.pone.0128687