PPIRE12255

Target Protein Information
Protein_Name Streptavidin
Protein_Sequence MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ
Organism_Source Streptomyces avidinii
Functional_Classification avidin family
Cellular_Localization Extracellular
Gene_Names None
UniProt_ID P22629
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name cyclo-Ac-AE[CHPQGPPC]IEGRK-NH2
Peptide_Sequence AECHPQGPPCIEGRK
Peptide_Length 15
Peptide_SMILES CC[C@H](C)[C@H](NC(=O)[C@H](CS)NC(=O)[C@@H]1CCCN1C(=O)[C@@H]1CCCN1C(=O)CNC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CS)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](C)N)C(=O)N[C@@H](CCC(=O)O)C(=O)NCC(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCCN)C(=O)O
Chemical_Modification None
Cyclization_Method Side chain-side chain cyclization; C3<->C10; disulfide bond
Linear/Cyclic Cyclic
N-terminal_Modification Acetyl
C-terminal_Modification amide
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1621.85
Aliphatic_Index 32.66667
Aromaticity 0.00000
Average_Rotatable_Bonds 3.26667
Charge_at_pH_7 -0.03180
Isoelectric_Point 7.25494
Number_of_Hydrogen_Bond_Acceptors 24
Number_of_Hydrogen_Bond_Donors 23
Topological_Polar_Surface_Area 678.64000
X_logP_energy -7.75433
Interaction Information
Affinity KD=130 nM
Affinity_Assay Surface plasmon resonance
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title In crystals of complexes of streptavidin with peptide ligands containing the HPQ sequence the pKa of the peptide histidine is less than 3.0.
Release_Year 1997
PMID 9148939
DOI 10.1074/jbc.272.20.13220