PPIRE12510

Target Protein Information
Protein_Name Neuronal acetylcholine receptor subunit alpha-3
Protein_Sequence MGVVLLPPPLSMLMLVLMLLPAASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRPTSGEGDTPKTRTFYGAELSNLNCFSRADSKSCKEGYPCQDGTCGYCHHRRVKISNFSANLTRSSSSESVNAVLSLSALSPEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPLMARDDT
Organism_Source Rattus norvegicus
Functional_Classification ligand-gated ion channels
Cellular_Localization Plasma membrane
Gene_Names Chrna3
UniProt_ID P04757
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name R-Conotoxin MII E11A
Peptide_Sequence GCCSNPVCHLAHSNLC
Peptide_Length 16
Peptide_SMILES CC(C)C[C@H](NC(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CS)NC(=O)[C@@H](NC(=O)[C@@H]1CCCN1C(=O)[C@H](CC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CS)NC(=O)[C@H](CS)NC(=O)CN)C(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CS)C(=O)O
Chemical_Modification None
Cyclization_Method Side chain-side chain cyclization; C2<->C8; disulfide bond; C3<->C16; disulfide bond
Linear/Cyclic Cyclic
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1657.92
Aliphatic_Index 73.12500
Aromaticity 0.00000
Average_Rotatable_Bonds 3.06250
Charge_at_pH_7 -0.06810
Isoelectric_Point 7.29026
Number_of_Hydrogen_Bond_Acceptors 27
Number_of_Hydrogen_Bond_Donors 26
Topological_Polar_Surface_Area 675.03000
X_logP_energy -10.03780
Interaction Information
Affinity IC50=16.9 nM
Affinity_Assay two-electrode voltage clamp electrophysiology
PDB_ID None
Type Ion channel modulator
Structure
Reference Information
Document_Type Research Articles
Title Determinants of potency on alpha-conotoxin MII, a peptide antagonist of neuronal nicotinic receptors.
Release_Year 2004
PMID 15005608
DOI 10.1021/bi036180h