PPIRE12851

Target Protein Information
Protein_Name Histone chaperone ASF1
Protein_Sequence MSIVSLLGIKVLNNPAKFTDPYEFEITFECLESLKHDLEWKLTYVGSSRSLDHDQELDSILVGPVPVGVNKFVFSADPPSAELIPASELVSVTVILLSCSYDGREFVRVGYYVNNEYDEEELRENPPAKVQVDHIVRNILAEKPRVTRFNIVWDNENEGDLYPPEQPGVDDEEEEDDEEEDDDEDDEDDEDDDQEDGEGEAEEAAEEEEEEEEKTEDNETNLEEEEEDIENSDGDEEEGEEEVGSVDKNEDGNDKKRRKIEGGSTDIESTPKDAARSTN
Organism_Source Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Functional_Classification histone chaperone
Cellular_Localization Nucleus
Gene_Names ASF1
UniProt_ID P32447
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name Rtt109419-436
Peptide_Sequence LAITMLKPRKKAKALPKT
Peptide_Length 18
Peptide_SMILES CC[C@H](C)[C@H](NC(=O)[C@H](C)NC(=O)[C@@H](N)CC(C)C)C(=O)N[C@H](C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCCN)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](C)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCCCN)C(=O)N[C@H](C(=O)O)[C@@H](C)O)[C@@H](C)O
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 2008.58
Aliphatic_Index 103.33333
Aromaticity 0.00000
Average_Rotatable_Bonds 3.88889
Charge_at_pH_7 5.99651
Isoelectric_Point 12.05455
Number_of_Hydrogen_Bond_Acceptors 28
Number_of_Hydrogen_Bond_Donors 27
Topological_Polar_Surface_Area 772.90000
X_logP_energy -4.68063
Interaction Information
Affinity KD=6.91 uM
Affinity_Assay Isothermal titration calorimetry
PDB_ID 6F0Y
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Structural characterization of the Asf1-Rtt109 interaction and its role in histone acetylation.
Release_Year 2017
PMID 29300933
DOI 10.1093/nar/gkx1283