PPIRE12943

Target Protein Information
Protein_Name Chemotaxis protein CheY
Protein_Sequence MADKELKFLVVDDFSTMRRIVRNLLKELGFNNVEEAEDGVDALNKLQAGGYGFVISDWNMPNMDGLELLKTIRADGAMSALPVLMVTAEAKKENIIAAAQAGASGYVVKPFTAATLEEKLNKIFEKLGM
Organism_Source Escherichia coli (strain K12)
Functional_Classification response regulators
Cellular_Localization Cytoplasm
Gene_Names cheY
UniProt_ID P0AE67
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name CheZ peptide
Peptide_Sequence AGVVASQDQVDDLLDSLGF
Peptide_Length 19
Peptide_SMILES CC(C)C[C@H](NC(=O)[C@H](CO)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](C)NC(=O)[C@@H](NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](C)N)C(C)C)C(C)C)C(C)C)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)O
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1949.10
Aliphatic_Index 117.89474
Aromaticity 0.05263
Average_Rotatable_Bonds 3.36842
Charge_at_pH_7 -4.00023
Isoelectric_Point 3.22803
Number_of_Hydrogen_Bond_Acceptors 28
Number_of_Hydrogen_Bond_Donors 28
Topological_Polar_Surface_Area 862.96000
X_logP_energy -9.80930
Interaction Information
Affinity KD=42 uM
Affinity_Assay Isothermal titration calorimetry
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title The structures of T87I phosphono-CheY and T87I/Y106W phosphono-CheY help to explain their binding affinities to the FliM and CheZ peptides.
Release_Year 2008
PMID 18801331
DOI 10.1016/j.abb.2008.08.019