PPIRE12961

Target Protein Information
Protein_Name von Hippel-Lindau disease tumor suppressor
Protein_Sequence MPRRAENWDEAEVGAEEAGVEEYGPEEDGGEESGAEESGPEESGPEELGAEEEMEAGRPRPVLRSVNSREPSQVIFCNRSPRVVLPVWLNFDGEPQPYPTLPPGTGRRIHSYRGHLWLFRDAGTHDGLLVNQTELFVPSLNVDGQPIFANITLPVYTLKERCLQVVRSLVKPENYRRLDIVRSLYEDLEDHPNVQKDLERLTQERIAHQRMGD
Organism_Source Homo sapiens
Functional_Classification E3 ubiquitin ligases
Cellular_Localization Cytoplasm
Gene_Names VHL
UniProt_ID P40337
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name HIF-1alpha CODD peptide 13c
Peptide_Sequence DEALAXYIPMDDDFQLRSF
Peptide_Length 19
Peptide_SMILES CC[C@H](C)[C@H](NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)CNC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H](N)CC(=O)O)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCSC)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1ccccc1)C(=O)O
Chemical_Modification X6=2S,4R-4-hydroxyproline
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification amide
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 2203.41
Aliphatic_Index 72.10526
Aromaticity 0.15789
Average_Rotatable_Bonds 3.68421
Charge_at_pH_7 -3.99931
Isoelectric_Point 3.57491
Number_of_Hydrogen_Bond_Acceptors 30
Number_of_Hydrogen_Bond_Donors 30
Topological_Polar_Surface_Area 910.28000
X_logP_energy -7.08813
Interaction Information
Affinity KD=11 nM
Affinity_Assay Isothermal titration calorimetry
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title 3-Fluoro-4-hydroxyprolines: Synthesis, Conformational Analysis, and Stereoselective Recognition by the VHL E3 Ubiquitin Ligase for Targeted Protein Degradation.
Release_Year 2018
PMID 29949369
DOI 10.1021/jacs.8b05807