PPIRE13488

Target Protein Information
Protein_Name Neuronal acetylcholine receptor subunit alpha-3
Protein_Sequence MGVVLLPPPLSMLMLVLMLLPAASASEAEHRLFQYLFEDYNEIIRPVANVSHPVIIQFEVSMSQLVKVDEVNQIMETNLWLKQIWNDYKLKWKPSDYQGVEFMRVPAEKIWKPDIVLYNNADGDFQVDDKTKALLKYTGEVTWIPPAIFKSSCKIDVTYFPFDYQNCTMKFGSWSYDKAKIDLVLIGSSMNLKDYWESGEWAIIKAPGYKHEIKYNCCEEIYQDITYSLYIRRLPLFYTINLIIPCLLISFLTVLVFYLPSDCGEKVTLCISVLLSLTVFLLVITETIPSTSLVIPLIGEYLLFTMIFVTLSIVITVFVLNVHYRTPTTHTMPTWVKAVFLNLLPRVMFMTRPTSGEGDTPKTRTFYGAELSNLNCFSRADSKSCKEGYPCQDGTCGYCHHRRVKISNFSANLTRSSSSESVNAVLSLSALSPEIKEAIQSVKYIAENMKAQNVAKEIQDDWKYVAMVIDRIFLWVFILVCILGTAGLFLQPLMARDDT
Organism_Source Rattus norvegicus
Functional_Classification ligand-gated ion channels
Cellular_Localization Plasma membrane
Gene_Names Chrna3
UniProt_ID P04757
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name Bovine catestatin
Peptide_Sequence RSMRLSFRARGYGFRGPGPQL
Peptide_Length 21
Peptide_SMILES CSCC[C@H](NC(=O)[C@H](CO)NC(=O)[C@@H](N)CCCNC(=N)N)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)N[C@@H](Cc1ccc(O)cc1)C(=O)NCC(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)N1CCC[C@H]1C(=O)NCC(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)O
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 2409.80
Aliphatic_Index 41.90476
Aromaticity 0.14286
Average_Rotatable_Bonds 3.66667
Charge_at_pH_7 4.99712
Isoelectric_Point 12.67708
Number_of_Hydrogen_Bond_Acceptors 32
Number_of_Hydrogen_Bond_Donors 39
Topological_Polar_Surface_Area 1041.02000
X_logP_energy -11.50235
Interaction Information
Affinity IC50=0.24 uM
Affinity_Assay radiolabeled sodium uptake assay
PDB_ID None
Type antagonist
Structure
Reference Information
Document_Type Research Articles
Title Desensitization of catecholamine release. The novel catecholamine release-inhibitory peptide catestatin (chromogranin a344-364)acts at the receptor to prevent nicotinic cholinergic tolerance.
Release_Year 1999
PMID 9915830
DOI 10.1074/jbc.274.5.2920