PPIRE13883

Target Protein Information
Protein_Name Kinetochore protein SPC24/Kinetochore protein SPC25
Protein_Sequence MSQKDNLLDNPVEFLKEVRESFDIQQDVDAMKRIRHDLDVIKEESEARISKEHSKVSESNKKLNAERINVAKLEGDLEYTNEESNEFGSKDELVKLLKDLDGLERNIVSLRSELDEKMKLYLKDSEIISTPNGSKIKAKVIEPELEEQSAVTPEANENILKLKLYRSLGVILDLENDQVLINRKNDGNIDILPLDNNLSDFYKTKYIWERLGK/MASIDAFSDLERRMDGFQKDVAQVLARQQNHARQQLQQFQAEMRQLHNQHQHLIDELQRLATQRTALQQQIHAAQQATNTTREQWRSYHERESELSRRQSTLAAQSRELDSLLQQRGKECVQLRARWAAQSGNDAAEVALYERLLQLRVLPGASDVHDVRFVFGDDSRCWIEVAMHGDHVIGNSHPALDPKSRATLEHVLTVQGDLAAFLVVARDMLLASL
Organism_Source Saccharomyces cerevisiae (strain ATCC 204508 / S288c)/Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Functional_Classification kinetochore proteins
Cellular_Localization Nucleus
Gene_Names SPC24/SPC25
UniProt_ID Q04477/P40014
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name Cnn1(60-84)
Peptide_Sequence NKDPNEVRSFLQDLSQVLARKSQGN
Peptide_Length 25
Peptide_SMILES CC(C)C[C@H](NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CO)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCCCN)NC(=O)[C@@H](N)CC(N)=O)C(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CO)C(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)O)C(C)C
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 2844.14
Aliphatic_Index 74.00000
Aromaticity 0.04000
Average_Rotatable_Bonds 3.96000
Charge_at_pH_7 1.00006
Isoelectric_Point 9.53242
Number_of_Hydrogen_Bond_Acceptors 42
Number_of_Hydrogen_Bond_Donors 45
Topological_Polar_Surface_Area 1359.90000
X_logP_energy -18.11766
Interaction Information
Affinity KD=3.5 uM
Affinity_Assay Isothermal titration calorimetry
PDB_ID 4GEQ
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title A structural basis for kinetochore recruitment of the Ndc80 complex via two distinct centromere receptors.
Release_Year 2013
PMID 23334295
DOI 10.1038/emboj.2012.356