PPIRE14101

Target Protein Information
Protein_Name Guanine nucleotide-binding protein G(i)subunit alpha-1
Protein_Sequence MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDAARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
Organism_Source Rattus norvegicus
Functional_Classification GTPases
Cellular_Localization Plasma membrane
Gene_Names Gnai1
UniProt_ID P10824
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name GPR consensus
Peptide_Sequence TMGEEDFFDLLAKSQSKRMDDQRVDLAG
Peptide_Length 28
Peptide_SMILES CSCC[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CO)NC(=O)[C@H](CCCCN)NC(=O)[C@H](C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)CNC(=O)[C@H](CCSC)NC(=O)[C@@H](N)[C@@H](C)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@H](C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](C)C(=O)NCC(=O)O)C(C)C
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification amide
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 3203.55
Aliphatic_Index 59.28571
Aromaticity 0.07143
Average_Rotatable_Bonds 4.03571
Charge_at_pH_7 -2.99683
Isoelectric_Point 4.14097
Number_of_Hydrogen_Bond_Acceptors 47
Number_of_Hydrogen_Bond_Donors 49
Topological_Polar_Surface_Area 1432.83000
X_logP_energy -15.63956
Interaction Information
Affinity KD=1.02 uM
Affinity_Assay Isothermal titration calorimetry
PDB_ID None
Type Inhibitor
Structure
Reference Information
Document_Type Research Articles
Title Thermodynamic characterization of the binding of activator of G protein signaling 3 (AGS3)and peptides derived from AGS3 with G alpha i1.
Release_Year 2003
PMID 14530282
DOI 10.1074/jbc.M306300200