PPIRE14600

Target Protein Information
Protein_Name Guanine nucleotide-binding protein G(i)subunit alpha-1
Protein_Sequence MGCTLSAEDKAAVERSKMIDRNLREDGEKAAREVKLLLLGAGESGKSTIVKQMKIIHEAGYSEEECKQYKAVVYSNTIQSIIAIIRAMGRLKIDFGDSARADDARQLFVLAGAAEEGFMTAELAGVIKRLWKDSGVQACFNRSREYQLNDSAAYYLNDLDRIAQPNYIPTQQDVLRTRVKTTGIVETHFTFKDLHFKMFDVGGQRSERKKWIHCFEGVTAIIFCVALSDYDLVLAEDEEMNRMHESMKLFDSICNNKWFTDTSIILFLNKKDLFEEKIKKSPLTICYPEYAGSNTYEEAAAYIQCQFEDLNKRKDTKEIYTHFTCATDTKNVQFVFDAVTDVIIKNNLKDCGLF
Organism_Source Homo sapiens
Functional_Classification GTPases
Cellular_Localization Plasma membrane
Gene_Names GNAI1
UniProt_ID P63096
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name FITC-RGS14 GoLoco motif peptide
Peptide_Sequence ASDIEGLVELLNRVQSSGAHDQRGLLRKEDLVLPEFLQ
Peptide_Length 38
Peptide_SMILES CC[C@H](C)[C@H](NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](C)N)C(=O)N[C@@H](CCC(=O)O)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@H](C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CO)C(=O)N[C@@H](CO)C(=O)NCC(=O)N[C@@H](C)C(=O)N[C@@H](Cc1c[nH]cn1)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCCNC(=N)N)C(=O)NCC(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@H](C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](Cc1ccccc1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)O)C(C)C)C(C)C)C(C)C
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Fitc
C-terminal_Modification amide
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 4246.79
Aliphatic_Index 120.52632
Aromaticity 0.02632
Average_Rotatable_Bonds 3.86842
Charge_at_pH_7 -2.90297
Isoelectric_Point 4.45279
Number_of_Hydrogen_Bond_Acceptors 58
Number_of_Hydrogen_Bond_Donors 63
Topological_Polar_Surface_Area 1865.78000
X_logP_energy -18.18159
Interaction Information
Affinity KD=2.3 nM
Affinity_Assay Fluorescence Polarization
PDB_ID 3ONW
Type Inhibitor
Structure
Reference Information
Document_Type Research Articles
Title Structural determinants of affinity enhancement between GoLoco motifs and G-protein alpha subunit mutants.
Release_Year 2011
PMID 21115486
DOI 10.1074/jbc.M110.190496