PPIRE14682

Target Protein Information
Protein_Name Spindle assembly checkpoint protein BUB3
Protein_Sequence MQIVQIEQAPKDYISDIKIIPSKSLLLITSWDGSLTVYKFDIQAKNVDLLQSLRYKHPLLCCNFIDNTDLQIYVGTVQGEILKVDLIGSPSFQALTNNEANLGICRICKYGDDKLIAASWDGLIEVIDPRNYGDGVIAVKNLNSNNTKVKNKIFTMDTNSSRLIVGMNNSQVQWFRLPLCEDDNGTIEESGLKYQIRDVALLPKEQEGYACSSIDGRVAVEFFDDQGDDYNSSKRFAFRCHRLNLKDTNLAYPVNSIEFSPRHKFLYTAGSDGIISCWNLQTRKKIKNFAKFNEDSVVKIACSDNILCLATSDDTFKTNAAIDQTIELNASSIYIIFDYEN
Organism_Source Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
Functional_Classification WD40 repeat proteins
Cellular_Localization Cytoplasm
Gene_Names BUB3
UniProt_ID P26449
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name Bub1 GLEBS motif peptide
Peptide_Sequence DEEELSELQELSDVEAQLRDELEQLQELDRLEELQELRDE
Peptide_Length 40
Peptide_SMILES CC(C)C[C@H](NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H](NC(=O)[C@H](CC(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@@H](N)CC(=O)O)C(C)C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(=O)N[C@@H](CCC(=O)O)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCCNC(=N)N)C(=O)N[C@@H](CC(=O)O)C(=O)N[C@@H](CCC(=O)O)C(=O)O
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 4857.14
Aliphatic_Index 107.25000
Aromaticity 0.00000
Average_Rotatable_Bonds 4.45000
Charge_at_pH_7 -14.97672
Isoelectric_Point 3.43583
Number_of_Hydrogen_Bond_Acceptors 69
Number_of_Hydrogen_Bond_Donors 75
Topological_Polar_Surface_Area 2311.23000
X_logP_energy -21.83979
Interaction Information
Affinity KD=3.6 uM
Affinity_Assay Isothermal titration calorimetry
PDB_ID 2I3T
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Structural analysis of Bub3 interactions in the mitotic spindle checkpoint.
Release_Year 2007
PMID 17227844
DOI 10.1073/pnas.0610358104