PPIRE15371
Target Protein Information
| Protein_Name | Protein farnesyltransferase/geranylgeranyltransferase type-1 subunit alpha |
|---|---|
| Protein_Sequence | MEEYDYSDVKPLPIETDLQDELCRIMYTEDYKRLMGLARALISLNELSPRALQLTAEIIDVAPAFYTIWNYRFNIVRHMMSESEDTVLYLNKELDWLDEVTLNNPKNYQIWSYRQSLLKLHPSPSFKRELPILKLMIDDDSKNYHVWSYRKWCCLFFSDFQHELAYASDLIETDIYNNSAWTHRMFYWVNAKDVISKVELADELQFIMDKIQLVPQNISPWTYLRGFQELFHDRLQWDSKVVDFATTFIGDVLSLPIGSPEDLPEIESSYALEFLAYHWGADPCTRDNAVKAYSLLAIKYDPIRKNLWHHKINNLN |
| Organism_Source | Saccharomyces cerevisiae (strain ATCC 204508 / S288c) |
| Functional_Classification | protein prenyltransferases |
| Cellular_Localization | Cytoplasm |
| Gene_Names | RAM2 |
| UniProt_ID | P29703 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | flRTRCVIA |
|---|---|
| Peptide_Sequence | RTRCVIA |
| Peptide_Length | 7 |
| Peptide_SMILES | CC[C@H](C)[C@H](NC(=O)[C@@H](NC(=O)[C@H](CS)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](NC(=O)[C@@H](N)CCCNC(=N)N)[C@@H](C)O)C(C)C)C(=O)N[C@@H](C)C(=O)O |
| Chemical_Modification | None |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | 27difluorofluorescein-5-carboxyl |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 818.00 |
|---|---|
| Aliphatic_Index | 111.42857 |
| Aromaticity | 0.00000 |
| Average_Rotatable_Bonds | 3.71429 |
| Charge_at_pH_7 | 1.93600 |
| Isoelectric_Point | 10.52641 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 12 |
| Number_of_Hydrogen_Bond_Donors | 16 |
| Topological_Polar_Surface_Area | 381.95000 |
| X_logP_energy | -4.13376 |
Interaction Information
| Affinity | KD=11 uM |
|---|---|
| Affinity_Assay | fluorescence anisotropy |
| PDB_ID | None |
| Type | Substrate |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Yeast Protein Farnesyltransferase. pKas of Peptide Substrates Bound as Zinc Thiolates |
| Release_Year | 1999 |
| PMID | 10529185 |
| DOI | 10.1021/bi990794y |