PPIRE15663

Target Protein Information
Protein_Name Streptavidin
Protein_Sequence MRKIVVAAIAVSLTTVSITASASADPSKDSKAQVSAAEAGITGTWYNQLGSTFIVTAGADGALTGTYESAVGNAESRYVLTGRYDSAPATDGSGTALGWTVAWKNNYRNAHSATTWSGQYVGGAEARINTQWLLTSGTTEANAWKSTLVGHDTFTKVKPSAASIDAAKKAGVNNGNPLDAVQQ
Organism_Source Streptomyces avidinii
Functional_Classification avidin family proteins
Cellular_Localization Extracellular
Gene_Names None
UniProt_ID P22629
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name SAHP (streptavidin-binding)
Peptide_Sequence WSHPQFEKGGGSWTWENGKWTWKGXXXXXX
Peptide_Length 30
Peptide_SMILES C[C@@H](O)[C@H](NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](CCCCN)NC(=O)CNC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@@H](NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](CO)NC(=O)CNC(=O)CNC(=O)CNC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(=O)O)NC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@@H]1CCCN1C(=O)[C@H](Cc1c[nH]cn1)NC(=O)[C@H](CO)NC(=O)[C@@H](N)Cc1c[nH]c2ccccc12)[C@@H](C)O)C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)N[C@@H](CCCCN)C(=O)NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(=O)NCC(=O)O
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 3219.44
Aliphatic_Index 0.00000
Aromaticity 0.20000
Average_Rotatable_Bonds 3.30000
Charge_at_pH_7 1.09156
Isoelectric_Point 9.44369
Number_of_Hydrogen_Bond_Acceptors 43
Number_of_Hydrogen_Bond_Donors 47
Topological_Polar_Surface_Area 1325.82000
X_logP_energy -14.25870
Interaction Information
Affinity KD=68 nM
Affinity_Assay Surface Plasmon Resonance
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Conversion of Low-Affinity Peptides to High-Affinity Peptide Binders by Using a b-Hairpin Scaffold-Assisted Approach
Release_Year 2014
PMID 25371172
DOI 10.1002/cbic.201402450