PPIRE16008

Target Protein Information
Protein_Name Gamma-aminobutyric acid receptor subunit beta-2
Protein_Sequence MWRVRKRGYFGIWSFPLIIAAVCAQSVNDPSNMSLVKETVDRLLKGYDIRLRPDFGGPPVAVGMNIDIASIDMVSEVNMDYTLTMYFQQAWRDKRLSYNVIPLNLTLDNRVADQLWVPDTYFLNDKKSFVHGVTVKNRMIRLHPDGTVLYGLRITTTAACMMDLRRYPLDEQNCTLEIESYGYTTDDIEFYWRGDDNAVTGVTKIELPQFSIVDYKLITKKVVFSTGSYPRLSLSFKLKRNIGYFILQTYMPSILITILSWVSFWINYDASAARVALGITTVLTMTTINTHLRETLPKIPYVKAIDMYLMGCFVFVFMALLEYALVNYIFFGRGPQRQKKAAEKAANANNEKMRLDVNKMDPHENILLSTLEIKNEMATSEAVMGLGDPRSTMLAYDASSIQYRKAGLPRHSFGRNALERHVAQKKSRLRRRASQLKITIPDLTDVNAIDRWSRIFFPVVFSFFNIVYWLYYVN
Organism_Source Rattus norvegicus
Functional_Classification ligand gated ion channels
Cellular_Localization Plasma membrane
Gene_Names Gabrb2
UniProt_ID P63138
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name GABAA b2 extracellular peptide
Peptide_Sequence MWRVRKRGYFGIWSFPLII
Peptide_Length 19
Peptide_SMILES CC[C@H](C)[C@H](NC(=O)[C@@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@@H]1CCCN1C(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](CO)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@@H](NC(=O)CNC(=O)[C@H](Cc1ccccc1)NC(=O)[C@H](Cc1ccc(O)cc1)NC(=O)CNC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@@H](N)CCSC)C(C)C)[C@@H](C)CC)[C@@H](C)CC)C(=O)O
Chemical_Modification None
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification biotin
C-terminal_Modification Amide
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 2425.97
Aliphatic_Index 97.36842
Aromaticity 0.26316
Average_Rotatable_Bonds 3.94737
Charge_at_pH_7 3.99683
Isoelectric_Point 12.22714
Number_of_Hydrogen_Bond_Acceptors 27
Number_of_Hydrogen_Bond_Donors 33
Topological_Polar_Surface_Area 862.09000
X_logP_energy -0.71999
Interaction Information
Affinity KD=0.86 uM
Affinity_Assay label-free biosensor (SRU BINDH)
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Generation of Recombinant Antibodies to Rat GABAA Receptor Subunits by Affinity Selection on Synthetic Peptides
Release_Year 2014
PMID 24586298
DOI 10.1371/journal.pone.0087964