PPIRE17362

Target Protein Information
Protein_Name None
Protein_Sequence MAWVWTLLFLMAAAQSIQAQIQLVQSGPELKKPGETVKISCKASGYTFTDYSMHWVKQAPGKGLKWMGWVNIETGESVYADDFKGRFAFSLETSASTIHLQINNLKNEDTATYFCARSDYDYDIYAMDYWGQGTSVTVSSESARNPTIYPLTLPPALSSDPVIIGCLIHDYFPSGTMNVTWGKSGKDITTVNFPPALASGGRYTMSSQLTLPAVECPEGESVKCSVQHDSNPVQELDVNCSGPTPPPPITIPSCQPSLSLQRPALEDLLLGSDASITCTLNGLRNPEGAVFTWEPSTGKDAVQKKAVQNSCGCYSVSSVLPGCAERWNSGASFKCTVTHPESGTLTGTIAKVTVNTFPPQVHLLPPPSEELALNELLSLTCLVRAFNPKEVLVRWLHGNEELSPESYLVFEPLKEPGEGATTYLVTSVLRVSAETWKQGDQYSCMVGHEALPMNFTQKTIDRLSGKPTNVSVSVIMSEGDGICY
Organism_Source Mus musculus
Functional_Classification antibodies
Cellular_Localization Extracellular
Gene_Names Igh
UniProt_ID Q99LA6
Protein-Protein Interaction Networks
Peptide Basic Information
Peptide_Name PCS(424-436-Tyr-430NO2)
Peptide_Sequence GKRLKNXSLPWGA
Peptide_Length 13
Peptide_SMILES CC(C)C[C@H](NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CCCCN)NC(=O)CN)C(=O)N[C@@H](CCCCN)C(=O)N[C@@H](CC(N)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(C)C)C(=O)N1CCC[C@H]1C(=O)N[C@@H](Cc1c[nH]c2ccccc12)C(=O)NCC(=O)N[C@@H](C)C(=O)O
Chemical_Modification X7=3-nitrotyrosine
Cyclization_Method None
Linear/Cyclic Linear
N-terminal_Modification Free
C-terminal_Modification Free
Amino_Acid_Distribution
Peptide Physicochemical
Molecular_Weight 1383.62
Aliphatic_Index 67.69231
Aromaticity 0.07692
Average_Rotatable_Bonds 3.46154
Charge_at_pH_7 2.99739
Isoelectric_Point 11.82306
Number_of_Hydrogen_Bond_Acceptors 19
Number_of_Hydrogen_Bond_Donors 21
Topological_Polar_Surface_Area 596.78000
X_logP_energy -6.15863
Interaction Information
Affinity KD=60 nM
Affinity_Assay Surface Plasmon Resonance
PDB_ID None
Type Affinity ligand
Structure
Reference Information
Document_Type Research Articles
Title Epitope motif of an anti-nitrotyrosine antibody specific for tyrosine-nitrated peptides revealed by a combination of affinity approaches and mass spectrometry
Release_Year 2011
PMID 21308874
DOI 10.1002/psc.1298