PPIRE18030
Target Protein Information
| Protein_Name | 4'-phosphopantetheinyl transferase Sfp |
|---|---|
| Protein_Sequence | MKIYGIYMDRPLSQEENERFMSFISPEKREKCRRFYHKEDAHRTLLGDVLVRSVISRQYQLDKSDIRFSTQEYGKPCIPDLPDAHFNISHSGRWVICAFDSQPIGIDIEKTKPISLEIAKRFFSKTEYSDLLAKDKDEQTDYFYHLWSMKESFIKQEGKGLSLPLDSFSVRLHQDGQVSIELPDSHSPCYIKTYEVDPGYKMAVCAAHPDFPEDITMVSYEELL |
| Organism_Source | Bacillus subtilis (strain 168) |
| Functional_Classification | phosphopantetheinyl transferases |
| Cellular_Localization | Cytoplasm |
| Gene_Names | sfp |
| UniProt_ID | P39135 |
| Protein-Protein Interaction Networks | |
Peptide Basic Information
| Peptide_Name | S6 |
|---|---|
| Peptide_Sequence | GDSLSWLLRLLN |
| Peptide_Length | 12 |
| Peptide_SMILES | CC(C)C[C@H](NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CCCNC(=N)N)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](Cc1c[nH]c2ccccc12)NC(=O)[C@H](CO)NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CO)NC(=O)[C@H](CC(=O)O)NC(=O)CN)C(=O)N[C@@H](CC(N)=O)C(=O)O |
| Chemical_Modification | None |
| Cyclization_Method | None |
| Linear/Cyclic | Linear |
| N-terminal_Modification | Free |
| C-terminal_Modification | Free |
| Amino_Acid_Distribution | |
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Peptide Physicochemical
| Molecular_Weight | 1386.61 |
|---|---|
| Aliphatic_Index | 162.50000 |
| Aromaticity | 0.08333 |
| Average_Rotatable_Bonds | 3.75000 |
| Charge_at_pH_7 | -0.00157 |
| Isoelectric_Point | 6.33872 |
|---|---|
| Number_of_Hydrogen_Bond_Acceptors | 18 |
| Number_of_Hydrogen_Bond_Donors | 21 |
| Topological_Polar_Surface_Area | 581.96000 |
| X_logP_energy | -4.08503 |
Interaction Information
| Affinity | Km=0.059 uM |
|---|---|
| Affinity_Assay | Enzyme Inhibition Kinetics |
| PDB_ID | None |
| Type | Substrate |
| Structure | |
Reference Information
| Document_Type | Research Articles |
|---|---|
| Title | Genetically Encoded Short Peptide Tags for Orthogonal Protein Labeling by Sfp and AcpS Phosphopantetheinyl Transferases |
| Release_Year | 2007 |
| PMID | 17465518 |
| DOI | 10.1021/cb700054k |